---
_id: '3753'
abstract:
- lang: eng
  text: Characterizing the dynamics of specific RNA levels requires real-time RNA
    profiling in a single cell. We show that the combination of a synthetic modular
    genetic system with fluorescence correlation spectroscopy allows us to directly
    measure in real time the activity of any specific promoter in prokaryotes. Using
    a simple inducible gene expression system, we found that induced RNA levels within
    a single bacterium of Escherichia coli exhibited a pulsating profile in response
    to a steady input of inducer. The genetic deletion of an efflux pump system, a
    key determinant of antibiotic resistance, altered the pulsating transcriptional
    dynamics and caused overexpression of induced RNA. In contrast with population
    measurements, real-time RNA profiling permits identifying relationships between
    genotypes and transcriptional dynamics that are accessible only at the level of
    the single cell.
acknowledgement: '4237'
author:
- first_name: Thuc
  full_name: Le,Thuc T.
  last_name: Le
- first_name: Sébastien
  full_name: Harlepp, Sébastien
  last_name: Harlepp
- first_name: Calin C
  full_name: Calin Guet
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
- first_name: Kimberly
  full_name: Dittmar,Kimberly
  last_name: Dittmar
- first_name: Thierry
  full_name: Emonet,Thierry
  last_name: Emonet
- first_name: Tao
  full_name: Pan,Tao
  last_name: Pan
- first_name: Philippe
  full_name: Cluzel,Philippe
  last_name: Cluzel
citation:
  ama: Le T, Harlepp S, Guet CC, et al. Real-time RNA profiling within a single bacterium.
    <i>PNAS</i>. 2005;102(26):9160-9164. doi:<a href="https://doi.org/10.1073/pnas.0503311102">10.1073/pnas.0503311102</a>
  apa: Le, T., Harlepp, S., Guet, C. C., Dittmar, K., Emonet, T., Pan, T., &#38; Cluzel,
    P. (2005). Real-time RNA profiling within a single bacterium. <i>PNAS</i>. National
    Academy of Sciences. <a href="https://doi.org/10.1073/pnas.0503311102">https://doi.org/10.1073/pnas.0503311102</a>
  chicago: Le, Thuc, Sébastien Harlepp, Calin C Guet, Kimberly Dittmar, Thierry Emonet,
    Tao Pan, and Philippe Cluzel. “Real-Time RNA Profiling within a Single Bacterium.”
    <i>PNAS</i>. National Academy of Sciences, 2005. <a href="https://doi.org/10.1073/pnas.0503311102">https://doi.org/10.1073/pnas.0503311102</a>.
  ieee: T. Le <i>et al.</i>, “Real-time RNA profiling within a single bacterium,”
    <i>PNAS</i>, vol. 102, no. 26. National Academy of Sciences, pp. 9160–9164, 2005.
  ista: Le T, Harlepp S, Guet CC, Dittmar K, Emonet T, Pan T, Cluzel P. 2005. Real-time
    RNA profiling within a single bacterium. PNAS. 102(26), 9160–9164.
  mla: Le, Thuc, et al. “Real-Time RNA Profiling within a Single Bacterium.” <i>PNAS</i>,
    vol. 102, no. 26, National Academy of Sciences, 2005, pp. 9160–64, doi:<a href="https://doi.org/10.1073/pnas.0503311102">10.1073/pnas.0503311102</a>.
  short: T. Le, S. Harlepp, C.C. Guet, K. Dittmar, T. Emonet, T. Pan, P. Cluzel, PNAS
    102 (2005) 9160–9164.
date_created: 2018-12-11T12:04:59Z
date_published: 2005-01-01T00:00:00Z
date_updated: 2021-01-12T07:51:57Z
day: '01'
doi: 10.1073/pnas.0503311102
extern: 1
intvolume: '       102'
issue: '26'
month: '01'
page: 9160 - 9164
publication: PNAS
publication_status: published
publisher: National Academy of Sciences
publist_id: '2476'
quality_controlled: 0
status: public
title: Real-time RNA profiling within a single bacterium
type: journal_article
volume: 102
year: '2005'
...