---
res:
  bibo_abstract:
  - Fluorescence correlation spectroscopy (FCS) has permitted the characterization
    of high concentrations of noncoding RNAs in a single living bacterium. Here, we
    extend the use of FCS to low concentrations of coding RNAs in single living cells.
    We genetically fuse a red fluorescent protein (RFP) gene and two binding sites
    for an RNA-binding protein, whose translated product is the RFP protein alone.
    Using this construct, we determine in single cells both the absolute [mRNA] concentration
    and the associated [RFP] expressed from an inducible plasmid. We find that the
    FCS method allows us to reliably monitor in real-time [mRNA] down to similar to
    40 nM (i.e. approximately two transcripts per volume of detection). To validate
    these measurements, we show that [mRNA] is proportional to the associated expression
    of the RFP protein. This FCS-based technique establishes a framework for minimally
    invasive measurements of mRNA concentration in individual living bacteria.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Calin C
      foaf_name: Guet, Calin C
      foaf_surname: Guet
      foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6220-2052
  - foaf_Person:
      foaf_givenName: Luke
      foaf_name: Bruneaux, Luke
      foaf_surname: Bruneaux
  - foaf_Person:
      foaf_givenName: Taejin
      foaf_name: Min, Taejin
      foaf_surname: Min
  - foaf_Person:
      foaf_givenName: Dan
      foaf_name: Siegal Gaskins, Dan
      foaf_surname: Siegal Gaskins
  - foaf_Person:
      foaf_givenName: Israel
      foaf_name: Figueroa, Israel
      foaf_surname: Figueroa
  - foaf_Person:
      foaf_givenName: Thierry
      foaf_name: Emonet, Thierry
      foaf_surname: Emonet
  - foaf_Person:
      foaf_givenName: Philippe
      foaf_name: Cluzel, Philippe
      foaf_surname: Cluzel
  bibo_doi: 10.1093/nar/gkn329
  bibo_issue: '12'
  bibo_volume: 36
  dct_date: 2008^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0305-1048
  - http://id.crossref.org/issn/1362-4962
  dct_language: eng
  dct_publisher: Oxford University Press@
  dct_title: Minimally invasive determination of mRNA concentration in single living
    bacteria@
  fabio_hasPubmedId: '18515347'
...