---
OA_place: publisher
OA_type: gold
_id: '3754'
abstract:
- lang: eng
  text: Fluorescence correlation spectroscopy (FCS) has permitted the characterization
    of high concentrations of noncoding RNAs in a single living bacterium. Here, we
    extend the use of FCS to low concentrations of coding RNAs in single living cells.
    We genetically fuse a red fluorescent protein (RFP) gene and two binding sites
    for an RNA-binding protein, whose translated product is the RFP protein alone.
    Using this construct, we determine in single cells both the absolute [mRNA] concentration
    and the associated [RFP] expressed from an inducible plasmid. We find that the
    FCS method allows us to reliably monitor in real-time [mRNA] down to similar to
    40 nM (i.e. approximately two transcripts per volume of detection). To validate
    these measurements, we show that [mRNA] is proportional to the associated expression
    of the RFP protein. This FCS-based technique establishes a framework for minimally
    invasive measurements of mRNA concentration in individual living bacteria.
acknowledgement: 'PMCID: PMC2475643 '
article_number: e73
article_processing_charge: No
article_type: original
author:
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
- first_name: Luke
  full_name: Bruneaux, Luke
  last_name: Bruneaux
- first_name: Taejin
  full_name: Min, Taejin
  last_name: Min
- first_name: Dan
  full_name: Siegal Gaskins, Dan
  last_name: Siegal Gaskins
- first_name: Israel
  full_name: Figueroa, Israel
  last_name: Figueroa
- first_name: Thierry
  full_name: Emonet, Thierry
  last_name: Emonet
- first_name: Philippe
  full_name: Cluzel, Philippe
  last_name: Cluzel
citation:
  ama: Guet CC, Bruneaux L, Min T, et al. Minimally invasive determination of mRNA
    concentration in single living bacteria. <i>Nucleic Acids Research</i>. 2008;36(12).
    doi:<a href="https://doi.org/10.1093/nar/gkn329">10.1093/nar/gkn329</a>
  apa: Guet, C. C., Bruneaux, L., Min, T., Siegal Gaskins, D., Figueroa, I., Emonet,
    T., &#38; Cluzel, P. (2008). Minimally invasive determination of mRNA concentration
    in single living bacteria. <i>Nucleic Acids Research</i>. Oxford University Press.
    <a href="https://doi.org/10.1093/nar/gkn329">https://doi.org/10.1093/nar/gkn329</a>
  chicago: Guet, Calin C, Luke Bruneaux, Taejin Min, Dan Siegal Gaskins, Israel Figueroa,
    Thierry Emonet, and Philippe Cluzel. “Minimally Invasive Determination of MRNA
    Concentration in Single Living Bacteria.” <i>Nucleic Acids Research</i>. Oxford
    University Press, 2008. <a href="https://doi.org/10.1093/nar/gkn329">https://doi.org/10.1093/nar/gkn329</a>.
  ieee: C. C. Guet <i>et al.</i>, “Minimally invasive determination of mRNA concentration
    in single living bacteria,” <i>Nucleic Acids Research</i>, vol. 36, no. 12. Oxford
    University Press, 2008.
  ista: Guet CC, Bruneaux L, Min T, Siegal Gaskins D, Figueroa I, Emonet T, Cluzel
    P. 2008. Minimally invasive determination of mRNA concentration in single living
    bacteria. Nucleic Acids Research. 36(12), e73.
  mla: Guet, Calin C., et al. “Minimally Invasive Determination of MRNA Concentration
    in Single Living Bacteria.” <i>Nucleic Acids Research</i>, vol. 36, no. 12, e73,
    Oxford University Press, 2008, doi:<a href="https://doi.org/10.1093/nar/gkn329">10.1093/nar/gkn329</a>.
  short: C.C. Guet, L. Bruneaux, T. Min, D. Siegal Gaskins, I. Figueroa, T. Emonet,
    P. Cluzel, Nucleic Acids Research 36 (2008).
date_created: 2018-12-11T12:04:59Z
date_published: 2008-05-30T00:00:00Z
date_updated: 2026-05-29T11:58:14Z
day: '30'
doi: 10.1093/nar/gkn329
extern: '1'
external_id:
  pmid:
  - '18515347'
fulldoi: https://doi.org/10.1093/nar/gkn329
intvolume: '        36'
issue: '12'
language:
- iso: eng
month: '05'
oa_version: Published Version
pmid: 1
publication: Nucleic Acids Research
publication_identifier:
  eissn:
  - 1362-4962
  issn:
  - 0305-1048
publication_status: published
publisher: Oxford University Press
publist_id: '2474'
status: public
title: Minimally invasive determination of mRNA concentration in single living bacteria
tmp:
  image: /images/cc_by_nc.png
  legal_code_url: https://creativecommons.org/licenses/by-nc/4.0/legalcode
  name: Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
  short: CC BY-NC (4.0)
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 36
year: '2008'
...