---
_id: '3755'
abstract:
- lang: eng
text: A primitive example of adaptation in gene expression is the balance between
the rate of synthesis and degradation of cellular RNA, which allows rapid responses
to environmental signals. Here, we investigate how multidrug efflux pump systems
mediate the dynamics of a simple drug-inducible system in response to a steady
level of inducer. Using fluorescence correlation spectroscopy, we measured in
real time within a single bacterium the transcription activity at the RNA level
of the acrAB-TolC multidrug efflux pump system. When cells are exposed to constant
level of anhydrotetracycline inducer and are adsorbed onto a poly-L-lysine-coated
surface, we found that the acrAB-TolC promoter is steadily active. We also monitored
the activity of the tet promoter to characterize the effect of this efflux system
on the dynamics of drug-inducible transcription. We found that the transcriptional
response of the tet promoter to a steady level of aTc rises and then falls back
to its preinduction level. The rate of RNA degradation was constant throughout
the transcriptional pulse, indicating that the modulation of intracellular inducer
concentration alone can produce this pulsating response. Single-cell experiments
together with numerical simulations suggest that such pulsating response in drug-inducible
genetic systems is a property emerging from the dependence of drug-inducible transcription
on multidrug efflux systems.
author:
- first_name: Thuc
full_name: Le,Thuc T.
last_name: Le
- first_name: Thierry
full_name: Emonet,Thierry
last_name: Emonet
- first_name: Sébastien
full_name: Harlepp, Sébastien
last_name: Harlepp
- first_name: Calin C
full_name: Calin Guet
id: 47F8433E-F248-11E8-B48F-1D18A9856A87
last_name: Guet
orcid: 0000-0001-6220-2052
- first_name: Philippe
full_name: Cluzel,Philippe
last_name: Cluzel
citation:
ama: Le T, Emonet T, Harlepp S, Guet CC, Cluzel P. Dynamical determinants of drug-inducible
gene expression in a single bacterium. Biophysical Journal. 2006;90(9):3315-3321.
doi:10.1529/biophysj.105.073353
apa: Le, T., Emonet, T., Harlepp, S., Guet, C. C., & Cluzel, P. (2006). Dynamical
determinants of drug-inducible gene expression in a single bacterium. Biophysical
Journal. Biophysical Society. https://doi.org/10.1529/biophysj.105.073353
chicago: Le, Thuc, Thierry Emonet, Sébastien Harlepp, Calin C Guet, and Philippe
Cluzel. “Dynamical Determinants of Drug-Inducible Gene Expression in a Single
Bacterium.” Biophysical Journal. Biophysical Society, 2006. https://doi.org/10.1529/biophysj.105.073353.
ieee: T. Le, T. Emonet, S. Harlepp, C. C. Guet, and P. Cluzel, “Dynamical determinants
of drug-inducible gene expression in a single bacterium,” Biophysical Journal,
vol. 90, no. 9. Biophysical Society, pp. 3315–3321, 2006.
ista: Le T, Emonet T, Harlepp S, Guet CC, Cluzel P. 2006. Dynamical determinants
of drug-inducible gene expression in a single bacterium. Biophysical Journal.
90(9), 3315–3321.
mla: Le, Thuc, et al. “Dynamical Determinants of Drug-Inducible Gene Expression
in a Single Bacterium.” Biophysical Journal, vol. 90, no. 9, Biophysical
Society, 2006, pp. 3315–21, doi:10.1529/biophysj.105.073353.
short: T. Le, T. Emonet, S. Harlepp, C.C. Guet, P. Cluzel, Biophysical Journal 90
(2006) 3315–3321.
date_created: 2018-12-11T12:04:59Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:51:58Z
day: '01'
doi: 10.1529/biophysj.105.073353
extern: 1
intvolume: ' 90'
issue: '9'
main_file_link:
- open_access: '1'
url: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1432126/
month: '01'
oa: 1
page: 3315 - 3321
publication: Biophysical Journal
publication_status: published
publisher: Biophysical Society
publist_id: '2472'
quality_controlled: 0
status: public
title: Dynamical determinants of drug-inducible gene expression in a single bacterium
type: journal_article
volume: 90
year: '2006'
...