---
res:
  bibo_abstract:
  - 'In many eukaryotic cells going through M-phase, a bipolar spindle is formed by
    microtubules nucleated from centrosomes. These microtubules, in addition to being
    `''captured” by kinetochores, may be stabilized by chromatin in two different
    ways: short-range stabilization effects may affect microtubules in close contact
    with the chromatin, while long-range stabilization effects may `''guide” microtubule
    growth towards the chromatin (e.g., by introducing a diffusive gradient of an
    enzymatic activity that affects microtubule assembly). Here, we use both meiotic
    and mitotic extracts from Xenopus laevis eggs to study microtubule aster formation
    and microtubule dynamics in the presence of chromatin. In `''low-speed” meiotic
    extracts, in the presence of salmon sperm chromatin, we find that short-range
    stabilization effects lead to a strong anisotropy of the microtubule asters. Analysis
    of the dynamic parameters of microtubule growth shows that this anisotropy arises
    from a decrease in the catastrophe frequency, an increase in the rescue frequency
    and a decrease in the growth velocity. In this system we also find evidence for
    long-range `''guidance” effects, which lead to a weak anisotropy of the asters.
    Statistically relevant results on these long-range effects are obtained in `''high-speed”
    mitotic extracts in the presence of artificially constructed chromatin stripes.
    We find that aster anisotropy is biased in the direction of the chromatin and
    that the catastrophe frequency is reduced in its vicinity. In this system we also
    find a surprising dependence of the catastrophe and the rescue frequencies on
    the length of microtubules nucleated from centrosomes: the catastrophe frequency
    increases and the rescue frequency decreases with microtubule length.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Marileen
      foaf_name: Dogterom, Marileen
      foaf_surname: Dogterom
  - foaf_Person:
      foaf_givenName: M.
      foaf_name: Felix, M.
      foaf_surname: Felix
  - foaf_Person:
      foaf_givenName: Calin C
      foaf_name: Guet, Calin C
      foaf_surname: Guet
      foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-6220-2052
  - foaf_Person:
      foaf_givenName: Stanislas
      foaf_name: Leibler, Stanislas
      foaf_surname: Leibler
  bibo_doi: 'doi: 10.1083/jcb.133.1.125 '
  bibo_issue: '1'
  bibo_volume: 133
  dct_date: 1996^xs_gYear
  dct_isPartOf:
  - http://id.crossref.org/issn/0021-9525
  dct_language: eng
  dct_publisher: Rockefeller University Press@
  dct_title: Influence of M-phase chromatin on the anisotropy of microtubule asters@
  fabio_hasPubmedId: '8601601'
...