article published yes Calin C Guet author 47F8433E-F248-11E8-B48F-1D18A9856A870000-0001-6220-2052 Michael Elowitz author Weihong Hsing author Stanislas Leibler author A central problem in biology is determining how genes interact as parts of functional networks. Creation and analysis of synthetic networks, composed of well-characterized genetic elements, provide a framework for theoretical modeling. Here, with the use of a combinatorial method, a library of networks with varying connectivity was generated in Escherichia coli. These networks were composed of genes encoding the transcriptional regulators Lacl, TetR, and lambda Cl, as well as the corresponding promoters. They displayed phenotypic behaviors resembling binary logical circuits, with two chemical “inputs” and a fluorescent protein “output.” Within this simple system, diverse computational functions arose through changes in network connectivity. Combinatorial synthesis provides an alternative approach for studying biological networks, as well as an efficient method for producing diverse phenotypes in vivo. American Association for the Advancement of Science2002 eng 0036-8075 1202913310.1126/science.1067407 29655721466 - 1470 yes Guet, C. C., Elowitz, M., Hsing, W., &#38; Leibler, S. (2002). Combinatorial synthesis of genetic networks. <i>Science</i>. American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.1067407">https://doi.org/10.1126/science.1067407</a> C.C. Guet, M. Elowitz, W. Hsing, S. Leibler, Science 296 (2002) 1466–1470. C. C. Guet, M. Elowitz, W. Hsing, and S. Leibler, “Combinatorial synthesis of genetic networks,” <i>Science</i>, vol. 296, no. 5572. American Association for the Advancement of Science, pp. 1466–1470, 2002. Guet, Calin C., et al. “Combinatorial Synthesis of Genetic Networks.” <i>Science</i>, vol. 296, no. 5572, American Association for the Advancement of Science, 2002, pp. 1466–70, doi:<a href="https://doi.org/10.1126/science.1067407">10.1126/science.1067407</a>. Guet CC, Elowitz M, Hsing W, Leibler S. Combinatorial synthesis of genetic networks. <i>Science</i>. 2002;296(5572):1466-1470. doi:<a href="https://doi.org/10.1126/science.1067407">10.1126/science.1067407</a> Guet, Calin C, Michael Elowitz, Weihong Hsing, and Stanislas Leibler. “Combinatorial Synthesis of Genetic Networks.” <i>Science</i>. American Association for the Advancement of Science, 2002. <a href="https://doi.org/10.1126/science.1067407">https://doi.org/10.1126/science.1067407</a>. Guet CC, Elowitz M, Hsing W, Leibler S. 2002. Combinatorial synthesis of genetic networks. Science. 296(5572), 1466–1470. 37572018-12-11T12:05:00Z2023-07-11T12:48:53Z