---
_id: '3757'
abstract:
- lang: eng
  text: A central problem in biology is determining how genes interact as parts of
    functional networks. Creation and analysis of synthetic networks, composed of
    well-characterized genetic elements, provide a framework for theoretical modeling.
    Here, with the use of a combinatorial method, a library of networks with varying
    connectivity was generated in Escherichia coli. These networks were composed of
    genes encoding the transcriptional regulators Lacl, TetR, and lambda Cl, as well
    as the corresponding promoters. They displayed phenotypic behaviors resembling
    binary logical circuits, with two chemical “inputs” and a fluorescent protein
    “output.” Within this simple system, diverse computational functions arose through
    changes in network connectivity. Combinatorial synthesis provides an alternative
    approach for studying biological networks, as well as an efficient method for
    producing diverse phenotypes in vivo.
article_processing_charge: No
article_type: original
author:
- first_name: Calin C
  full_name: Guet, Calin C
  id: 47F8433E-F248-11E8-B48F-1D18A9856A87
  last_name: Guet
  orcid: 0000-0001-6220-2052
- first_name: Michael
  full_name: Elowitz, Michael
  last_name: Elowitz
- first_name: Weihong
  full_name: Hsing, Weihong
  last_name: Hsing
- first_name: Stanislas
  full_name: Leibler, Stanislas
  last_name: Leibler
citation:
  ama: Guet CC, Elowitz M, Hsing W, Leibler S. Combinatorial synthesis of genetic
    networks. <i>Science</i>. 2002;296(5572):1466-1470. doi:<a href="https://doi.org/10.1126/science.1067407">10.1126/science.1067407</a>
  apa: Guet, C. C., Elowitz, M., Hsing, W., &#38; Leibler, S. (2002). Combinatorial
    synthesis of genetic networks. <i>Science</i>. American Association for the Advancement
    of Science. <a href="https://doi.org/10.1126/science.1067407">https://doi.org/10.1126/science.1067407</a>
  chicago: Guet, Calin C, Michael Elowitz, Weihong Hsing, and Stanislas Leibler. “Combinatorial
    Synthesis of Genetic Networks.” <i>Science</i>. American Association for the Advancement
    of Science, 2002. <a href="https://doi.org/10.1126/science.1067407">https://doi.org/10.1126/science.1067407</a>.
  ieee: C. C. Guet, M. Elowitz, W. Hsing, and S. Leibler, “Combinatorial synthesis
    of genetic networks,” <i>Science</i>, vol. 296, no. 5572. American Association
    for the Advancement of Science, pp. 1466–1470, 2002.
  ista: Guet CC, Elowitz M, Hsing W, Leibler S. 2002. Combinatorial synthesis of genetic
    networks. Science. 296(5572), 1466–1470.
  mla: Guet, Calin C., et al. “Combinatorial Synthesis of Genetic Networks.” <i>Science</i>,
    vol. 296, no. 5572, American Association for the Advancement of Science, 2002,
    pp. 1466–70, doi:<a href="https://doi.org/10.1126/science.1067407">10.1126/science.1067407</a>.
  short: C.C. Guet, M. Elowitz, W. Hsing, S. Leibler, Science 296 (2002) 1466–1470.
date_created: 2018-12-11T12:05:00Z
date_published: 2002-05-24T00:00:00Z
date_updated: 2023-07-11T12:48:53Z
day: '24'
doi: 10.1126/science.1067407
extern: '1'
external_id:
  pmid:
  - '12029133'
intvolume: '       296'
issue: '5572'
language:
- iso: eng
month: '05'
oa_version: None
page: 1466 - 1470
pmid: 1
publication: Science
publication_identifier:
  issn:
  - 0036-8075
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '2471'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Combinatorial synthesis of genetic networks
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 296
year: '2002'
...