---
_id: '3804'
abstract:
- lang: eng
text: Kv3 channels are thought to be essential for the fast-spiking (FS) phenotype
in GABAergic interneurons, but how these channels confer the ability to generate
action potentials (APs) at high frequency is unknown. To address this question,
we developed a fast dynamic-clamp system (approximately 50 kHz) that allowed us
to add a Kv3 model conductance to CA1 oriens alveus (OA) interneurons in hippocampal
slices. Selective pharmacological block of Kv3 channels by 0.3 mm 4-aminopyridine
or 1 mm tetraethylammonium ions led to a marked broadening of APs during trains
of short stimuli and a reduction in AP frequency during 1 sec stimuli. The addition
of artificial Kv3 conductance restored the original AP pattern. Subtraction of
Kv3 conductance by dynamic clamp mimicked the effects of the blockers. Application
of artificial Kv3 conductance also led to FS in OA interneurons after complete
K+ channel block and even induced FS in hippocampal pyramidal neurons in the absence
of blockers. Adding artificial Kv3 conductance with altered deactivation kinetics
revealed a nonmonotonic relationship between mean AP frequency and deactivation
rate, with a maximum slightly above the original value. Insertion of artificial
Kv3 conductance with either lowered activation threshold or inactivation also
led to a reduction in the mean AP frequency. However, the mechanisms were distinct.
Shifting the activation threshold induced adaptation, whereas adding inactivation
caused frequency-dependent AP broadening. In conclusion, Kv3 channels are necessary
for the FS phenotype of OA interneurons, and several of their gating properties
appear to be optimized for high-frequency repetitive activity.
author:
- first_name: Cheng
full_name: Lien, Cheng-Chang
last_name: Lien
- first_name: Peter M
full_name: Peter Jonas
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Lien C, Jonas PM. Kv3 potassium conductance is necessary and kinetically optimized
for high-frequency action potential generation in hippocampal interneurons. Journal
of Neuroscience. 2003;23(6):2058-2068.
apa: Lien, C., & Jonas, P. M. (2003). Kv3 potassium conductance is necessary
and kinetically optimized for high-frequency action potential generation in hippocampal
interneurons. Journal of Neuroscience. Society for Neuroscience.
chicago: Lien, Cheng, and Peter M Jonas. “Kv3 Potassium Conductance Is Necessary
and Kinetically Optimized for High-Frequency Action Potential Generation in Hippocampal
Interneurons.” Journal of Neuroscience. Society for Neuroscience, 2003.
ieee: C. Lien and P. M. Jonas, “Kv3 potassium conductance is necessary and kinetically
optimized for high-frequency action potential generation in hippocampal interneurons,”
Journal of Neuroscience, vol. 23, no. 6. Society for Neuroscience, pp.
2058–68, 2003.
ista: Lien C, Jonas PM. 2003. Kv3 potassium conductance is necessary and kinetically
optimized for high-frequency action potential generation in hippocampal interneurons.
Journal of Neuroscience. 23(6), 2058–68.
mla: Lien, Cheng, and Peter M. Jonas. “Kv3 Potassium Conductance Is Necessary and
Kinetically Optimized for High-Frequency Action Potential Generation in Hippocampal
Interneurons.” Journal of Neuroscience, vol. 23, no. 6, Society for Neuroscience,
2003, pp. 2058–68.
short: C. Lien, P.M. Jonas, Journal of Neuroscience 23 (2003) 2058–68.
date_created: 2018-12-11T12:05:16Z
date_published: 2003-01-01T00:00:00Z
date_updated: 2021-01-12T07:52:19Z
day: '01'
extern: 1
intvolume: ' 23'
issue: '6'
month: '01'
page: 2058 - 68
publication: Journal of Neuroscience
publication_status: published
publisher: Society for Neuroscience
publist_id: '2406'
quality_controlled: 0
status: public
title: Kv3 potassium conductance is necessary and kinetically optimized for high-frequency
action potential generation in hippocampal interneurons
type: journal_article
volume: 23
year: '2003'
...