---
_id: '3818'
abstract:
- lang: eng
text: Rigorous analysis of synaptic transmission in the central nervous system requires
access to presynaptic terminals. However, cortical terminals have been largely
inaccessible to presynaptic patch-clamp recording, due to their small size. Using
improved patch-clamp techniques in brain slices, we recorded from mossy fiber
terminals in the CA3 region of the hippocampus, which have a diameter of 2-5 microm.
The major steps of improvement were the enhanced visibility provided by high-numerical
aperture objectives and infrared illumination, the development of vibratomes with
minimal vertical blade vibrations and the use of sucrose-based solutions for storage
and cutting. Based on these improvements, we describe a protocol that allows us
to routinely record from hippocampal mossy fiber boutons. Presynaptic recordings
can be obtained in slices from both rats and mice. Presynaptic recordings can
be also obtained in slices from transgenic mice in which terminals are labeled
with enhanced green fluorescent protein.
author:
- first_name: Josef
full_name: Bischofberger, Josef
last_name: Bischofberger
- first_name: Dominique
full_name: Engel, Dominique
last_name: Engel
- first_name: Liyi
full_name: Li, Liyi
last_name: Li
- first_name: Jörg
full_name: Geiger, Jörg R
last_name: Geiger
- first_name: Peter M
full_name: Peter Jonas
id: 353C1B58-F248-11E8-B48F-1D18A9856A87
last_name: Jonas
orcid: 0000-0001-5001-4804
citation:
ama: Bischofberger J, Engel D, Li L, Geiger J, Jonas PM. Patch-clamp recording from
mossy fiber terminals in hippocampal slices. Nature Protocols. 2006;1(4):2075-2081.
doi:10.1038/nprot.2006.312
apa: Bischofberger, J., Engel, D., Li, L., Geiger, J., & Jonas, P. M. (2006).
Patch-clamp recording from mossy fiber terminals in hippocampal slices. Nature
Protocols. Nature Publishing Group. https://doi.org/10.1038/nprot.2006.312
chicago: Bischofberger, Josef, Dominique Engel, Liyi Li, Jörg Geiger, and Peter
M Jonas. “Patch-Clamp Recording from Mossy Fiber Terminals in Hippocampal Slices.”
Nature Protocols. Nature Publishing Group, 2006. https://doi.org/10.1038/nprot.2006.312 .
ieee: J. Bischofberger, D. Engel, L. Li, J. Geiger, and P. M. Jonas, “Patch-clamp
recording from mossy fiber terminals in hippocampal slices,” Nature Protocols,
vol. 1, no. 4. Nature Publishing Group, pp. 2075–81, 2006.
ista: Bischofberger J, Engel D, Li L, Geiger J, Jonas PM. 2006. Patch-clamp recording
from mossy fiber terminals in hippocampal slices. Nature Protocols. 1(4), 2075–81.
mla: Bischofberger, Josef, et al. “Patch-Clamp Recording from Mossy Fiber Terminals
in Hippocampal Slices.” Nature Protocols, vol. 1, no. 4, Nature Publishing
Group, 2006, pp. 2075–81, doi:10.1038/nprot.2006.312 .
short: J. Bischofberger, D. Engel, L. Li, J. Geiger, P.M. Jonas, Nature Protocols
1 (2006) 2075–81.
date_created: 2018-12-11T12:05:20Z
date_published: 2006-01-01T00:00:00Z
date_updated: 2021-01-12T07:52:25Z
day: '01'
doi: '10.1038/nprot.2006.312 '
extern: 1
intvolume: ' 1'
issue: '4'
month: '01'
page: 2075 - 81
publication: Nature Protocols
publication_status: published
publisher: Nature Publishing Group
publist_id: '2392'
quality_controlled: 0
status: public
title: Patch-clamp recording from mossy fiber terminals in hippocampal slices
type: journal_article
volume: 1
year: '2006'
...