---
_id: '3930'
abstract:
- lang: eng
  text: 'An active involvement of blood-brain barrier endothelial cell basement membranes
    in development of inflammatory lesions in the central nervous system (CNS) has
    not been considered to date. Here we investigated the molecular composition and
    possible function of the extracellular matrix encountered by extravasating T lymphocytes
    during experimental autoimmune encephalomyelitis (EAE). Endothelial basement membranes
    contained laminin 8 (alpha4beta1gamma1) and/or 10 (alpha5beta1gamma1) and their
    expression was influenced by proinflammatory cytokines or angiostatic agents.
    T cells emigrating into the CNS during EAE encountered two biochemically distinct
    basement membranes, the endothelial (containing laminins 8 and 10) and the parenchymal
    (containing laminins 1 and 2) basement membranes. However, inflammatory cuffs
    occurred exclusively around endothelial basement membranes containing laminin
    8, whereas in the presence of laminin 10 no infiltration was detectable. In vitro
    assays using encephalitogenic T cell lines revealed adhesion to laminins 8 and
    10, whereas binding to laminins 1 and 2 could not be induced. Downregulation of
    integrin alpha6 on cerebral endothelium at sites of T cell infiltration, plus
    a high turnover of laminin 8 at these sites, suggested two possible roles for
    laminin 8 in the endothelial basement membrane: one at the level of the endothelial
    cells resulting in reduced adhesion and, thereby, increased penetrability of the
    monolayer; and secondly at the level of the T cells providing direct signals to
    the transmigrating cells.'
acknowledgement: The authors thank Stefanie Karosi for careful and critical reading
  of the manuscript and Monika Bruckner for expert technical assistance. We are particularly
  grateful to Winfried Neuhuber for help with confocal microscopy and interpretation
  of the data. This work was supported by Deutsche Forschungsgemeinschaft grants So285/1-3
  and So285/1-4 to L.M. Sorokin.
article_processing_charge: No
article_type: original
author:
- first_name: Michael K
  full_name: Sixt, Michael K
  id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
  last_name: Sixt
  orcid: 0000-0002-6620-9179
- first_name: Britta
  full_name: Engelhardt, Britta
  last_name: Engelhardt
- first_name: Friederike
  full_name: Pausch, Friederike
  last_name: Pausch
- first_name: Rupert
  full_name: Hallmann, Rupert
  last_name: Hallmann
- first_name: Olaf
  full_name: Wendler, Olaf
  last_name: Wendler
- first_name: Lydia
  full_name: Sorokin, Lydia
  last_name: Sorokin
citation:
  ama: Sixt MK, Engelhardt B, Pausch F, Hallmann R, Wendler O, Sorokin L. Endothelial
    cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment
    across the blood-brain barrier in experimental autoimmune encephalomyelitis. <i>Journal
    of Cell Biology</i>. 2001;153(5):933-946. doi:<a href="https://doi.org/10.1083/jcb.153.5.933
    ">10.1083/jcb.153.5.933 </a>
  apa: Sixt, M. K., Engelhardt, B., Pausch, F., Hallmann, R., Wendler, O., &#38; Sorokin,
    L. (2001). Endothelial cell laminin isoforms, laminins 8 and 10, play decisive
    roles in T cell recruitment across the blood-brain barrier in experimental autoimmune
    encephalomyelitis. <i>Journal of Cell Biology</i>. Rockefeller University Press.
    <a href="https://doi.org/10.1083/jcb.153.5.933 ">https://doi.org/10.1083/jcb.153.5.933
    </a>
  chicago: Sixt, Michael K, Britta Engelhardt, Friederike Pausch, Rupert Hallmann,
    Olaf Wendler, and Lydia Sorokin. “Endothelial Cell Laminin Isoforms, Laminins
    8 and 10, Play Decisive Roles in T Cell Recruitment across the Blood-Brain Barrier
    in Experimental Autoimmune Encephalomyelitis.” <i>Journal of Cell Biology</i>.
    Rockefeller University Press, 2001. <a href="https://doi.org/10.1083/jcb.153.5.933
    ">https://doi.org/10.1083/jcb.153.5.933 </a>.
  ieee: M. K. Sixt, B. Engelhardt, F. Pausch, R. Hallmann, O. Wendler, and L. Sorokin,
    “Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in
    T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis,”
    <i>Journal of Cell Biology</i>, vol. 153, no. 5. Rockefeller University Press,
    pp. 933–946, 2001.
  ista: Sixt MK, Engelhardt B, Pausch F, Hallmann R, Wendler O, Sorokin L. 2001. Endothelial
    cell laminin isoforms, laminins 8 and 10, play decisive roles in T cell recruitment
    across the blood-brain barrier in experimental autoimmune encephalomyelitis. Journal
    of Cell Biology. 153(5), 933–946.
  mla: Sixt, Michael K., et al. “Endothelial Cell Laminin Isoforms, Laminins 8 and
    10, Play Decisive Roles in T Cell Recruitment across the Blood-Brain Barrier in
    Experimental Autoimmune Encephalomyelitis.” <i>Journal of Cell Biology</i>, vol.
    153, no. 5, Rockefeller University Press, 2001, pp. 933–46, doi:<a href="https://doi.org/10.1083/jcb.153.5.933
    ">10.1083/jcb.153.5.933 </a>.
  short: M.K. Sixt, B. Engelhardt, F. Pausch, R. Hallmann, O. Wendler, L. Sorokin,
    Journal of Cell Biology 153 (2001) 933–946.
date_created: 2018-12-11T12:05:57Z
date_published: 2001-05-21T00:00:00Z
date_updated: 2023-05-11T12:19:36Z
day: '21'
doi: '10.1083/jcb.153.5.933 '
extern: '1'
external_id:
  pmid:
  - '11381080'
intvolume: '       153'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2174323/
month: '05'
oa: 1
oa_version: Published Version
page: 933 - 946
pmid: 1
publication: Journal of Cell Biology
publication_identifier:
  issn:
  - 0021-9525
publication_status: published
publisher: Rockefeller University Press
publist_id: '2198'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Endothelial cell laminin isoforms, laminins 8 and 10, play decisive roles in
  T cell recruitment across the blood-brain barrier in experimental autoimmune encephalomyelitis
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 153
year: '2001'
...