--- _id: '3937' abstract: - lang: eng text: Lymphocyte motility in lymph nodes is regulated by chemokines, but the contribution of integrins to this motility remains obscure. Here we examined lymphocyte migration over CCR7-binding chemokines that 'decorate' lymph node stroma. In a shear-free environment, surface-bound lymph node chemokines but not their soluble counterparts promoted robust and sustained T lymphocyte motility. The chemokine CCL21 induced compartmentalized clustering of the integrins LFA-1 and VLA-4 in motile lymphocytes, but both integrins remained nonadhesive to ligands on lymphocytes, dendritic cells and stroma. The application of shear stress to lymphocytes interacting with CCL21 and integrin ligands promoted robust integrin-mediated adhesion. Thus, lymph node chemokines that promote motility and strongly activate lymphocyte integrins under shear forces fail to stimulate stable integrin adhesiveness in extravascular shear-free environments. author: - first_name: Eilon full_name: Woolf, Eilon last_name: Woolf - first_name: Irina full_name: Grigorova, Irina last_name: Grigorova - first_name: Adi full_name: Sagiv, Adi last_name: Sagiv - first_name: Valentin full_name: Grabovsky, Valentin last_name: Grabovsky - first_name: Sara full_name: Feigelson, Sara W last_name: Feigelson - first_name: Ziv full_name: Shulman, Ziv last_name: Shulman - first_name: Tanja full_name: Hartmann, Tanja last_name: Hartmann - first_name: Michael K full_name: Michael Sixt id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87 last_name: Sixt orcid: 0000-0002-6620-9179 - first_name: Jason full_name: Cyster, Jason G last_name: Cyster - first_name: Ronen full_name: Alon, Ronen last_name: Alon citation: ama: Woolf E, Grigorova I, Sagiv A, et al. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces. Nature Immunology. 2007;8(10):1076-1085. doi:10.1038/ni1499 apa: Woolf, E., Grigorova, I., Sagiv, A., Grabovsky, V., Feigelson, S., Shulman, Z., … Alon, R. (2007). Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces. Nature Immunology. Nature Publishing Group. https://doi.org/10.1038/ni1499 chicago: Woolf, Eilon, Irina Grigorova, Adi Sagiv, Valentin Grabovsky, Sara Feigelson, Ziv Shulman, Tanja Hartmann, Michael K Sixt, Jason Cyster, and Ronen Alon. “Lymph Node Chemokines Promote Sustained T Lymphocyte Motility without Triggering Stable Integrin Adhesiveness in the Absence of Shear Forces.” Nature Immunology. Nature Publishing Group, 2007. https://doi.org/10.1038/ni1499. ieee: E. Woolf et al., “Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces,” Nature Immunology, vol. 8, no. 10. Nature Publishing Group, pp. 1076–1085, 2007. ista: Woolf E, Grigorova I, Sagiv A, Grabovsky V, Feigelson S, Shulman Z, Hartmann T, Sixt MK, Cyster J, Alon R. 2007. Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces. Nature Immunology. 8(10), 1076–1085. mla: Woolf, Eilon, et al. “Lymph Node Chemokines Promote Sustained T Lymphocyte Motility without Triggering Stable Integrin Adhesiveness in the Absence of Shear Forces.” Nature Immunology, vol. 8, no. 10, Nature Publishing Group, 2007, pp. 1076–85, doi:10.1038/ni1499. short: E. Woolf, I. Grigorova, A. Sagiv, V. Grabovsky, S. Feigelson, Z. Shulman, T. Hartmann, M.K. Sixt, J. Cyster, R. Alon, Nature Immunology 8 (2007) 1076–1085. date_created: 2018-12-11T12:05:59Z date_published: 2007-08-26T00:00:00Z date_updated: 2021-01-12T07:53:19Z day: '26' doi: 10.1038/ni1499 extern: 1 intvolume: ' 8' issue: '10' month: '08' page: 1076 - 1085 publication: Nature Immunology publication_status: published publisher: Nature Publishing Group publist_id: '2189' quality_controlled: 0 status: public title: Lymph node chemokines promote sustained T lymphocyte motility without triggering stable integrin adhesiveness in the absence of shear forces type: journal_article volume: 8 year: '2007' ...