{"title":"Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells","_id":"3949","date_created":"2018-12-11T12:06:03Z","page":"5801 - 5810","date_updated":"2021-01-12T07:53:24Z","volume":113,"day":"04","quality_controlled":0,"publication":"Blood","abstract":[{"text":"Adhesion and motility of mammalian leukocytes are essential requirements for innate and adaptive immune defense mechanisms. We show here that the guanine nucleotide exchange factor cytohesin-1, which had previously been demonstrated to be an important component of beta-2 integrin activation in lymphocytes, regulates the activation of the small GTPase RhoA in primary dendritic cells (DCs). Cytohesin-1 and RhoA are both required for the induction of chemokine-dependent conformational changes of the integrin beta-2 subunit of DCs during adhesion under physiological flow conditions. Furthermore, use of RNAi in murine bone marrow DCs (BM-DCs) revealed that interference with cytohesin-1 signaling impairs migration of wild-type dendritic cells in complex 3D environments and in vivo. This phenotype was not observed in the complete absence of integrins. We thus demonstrate an essential role of cytohesin-1/RhoA during ameboid migration in the presence of integrins and further suggest that DCs without integrins switch to a different migration mode.","lang":"eng"}],"intvolume":"       113","extern":1,"publisher":"American Society of Hematology","doi":"10.1182/blood-2008-08-176123","year":"2009","publist_id":"2177","status":"public","issue":"23","citation":{"apa":"Quast, T., Tappertzhofen, B., Schild, C., Grell, J., Czeloth, N., Förster, R., … Kolanus, W. (2009). Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells. <i>Blood</i>. American Society of Hematology. <a href=\"https://doi.org/10.1182/blood-2008-08-176123\">https://doi.org/10.1182/blood-2008-08-176123</a>","mla":"Quast, Thomas, et al. “Cytohesin-1 Controls the Activation of RhoA and Modulates Integrin-Dependent Adhesion and Migration of Dendritic Cells.” <i>Blood</i>, vol. 113, no. 23, American Society of Hematology, 2009, pp. 5801–10, doi:<a href=\"https://doi.org/10.1182/blood-2008-08-176123\">10.1182/blood-2008-08-176123</a>.","ista":"Quast T, Tappertzhofen B, Schild C, Grell J, Czeloth N, Förster R, Alon R, Fraemohs L, Dreck K, Weber C, Lämmermann T, Sixt MK, Kolanus W. 2009. Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells. Blood. 113(23), 5801–5810.","ieee":"T. Quast <i>et al.</i>, “Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells,” <i>Blood</i>, vol. 113, no. 23. American Society of Hematology, pp. 5801–5810, 2009.","short":"T. Quast, B. Tappertzhofen, C. Schild, J. Grell, N. Czeloth, R. Förster, R. Alon, L. Fraemohs, K. Dreck, C. Weber, T. Lämmermann, M.K. Sixt, W. Kolanus, Blood 113 (2009) 5801–5810.","chicago":"Quast, Thomas, Barbara Tappertzhofen, Cora Schild, Jessica Grell, Niklas Czeloth, Reinhold Förster, Ronen Alon, et al. “Cytohesin-1 Controls the Activation of RhoA and Modulates Integrin-Dependent Adhesion and Migration of Dendritic Cells.” <i>Blood</i>. American Society of Hematology, 2009. <a href=\"https://doi.org/10.1182/blood-2008-08-176123\">https://doi.org/10.1182/blood-2008-08-176123</a>.","ama":"Quast T, Tappertzhofen B, Schild C, et al. Cytohesin-1 controls the activation of RhoA and modulates integrin-dependent adhesion and migration of dendritic cells. <i>Blood</i>. 2009;113(23):5801-5810. doi:<a href=\"https://doi.org/10.1182/blood-2008-08-176123\">10.1182/blood-2008-08-176123</a>"},"publication_status":"published","author":[{"full_name":"Quast, Thomas","first_name":"Thomas","last_name":"Quast"},{"full_name":"Tappertzhofen, Barbara","last_name":"Tappertzhofen","first_name":"Barbara"},{"first_name":"Cora","last_name":"Schild","full_name":"Schild, Cora"},{"full_name":"Grell, Jessica","last_name":"Grell","first_name":"Jessica"},{"full_name":"Czeloth, Niklas","last_name":"Czeloth","first_name":"Niklas"},{"full_name":"Förster, Reinhold","first_name":"Reinhold","last_name":"Förster"},{"full_name":"Alon, Ronen","last_name":"Alon","first_name":"Ronen"},{"full_name":"Fraemohs, Line","last_name":"Fraemohs","first_name":"Line"},{"last_name":"Dreck","first_name":"Katrin","full_name":"Dreck, Katrin"},{"last_name":"Weber","first_name":"Christian","full_name":"Weber, Christian"},{"last_name":"Lämmermann","first_name":"Tim","full_name":"Lämmermann, Tim"},{"last_name":"Sixt","first_name":"Michael K","full_name":"Michael Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","orcid":"0000-0002-6620-9179"},{"first_name":"Waldemar","last_name":"Kolanus","full_name":"Kolanus, Waldemar"}],"month":"06","type":"journal_article","date_published":"2009-06-04T00:00:00Z"}