---
_id: '3954'
abstract:
- lang: eng
text: The leading front of a cell can either protrude as an actin-free membrane
bleb that is inflated by actomyosin-driven contractile forces, or as an actin-rich
pseudopodium, a site where polymerizing actin filaments push out the membrane.
Pushing filaments can only cause the membrane to protrude if the expanding actin
network experiences a retrograde counter-force, which is usually provided by transmembrane
receptors of the integrin family. Here we show that chemotactic dendritic cells
mechanically adapt to the adhesive properties of their substrate by switching
between integrin-mediated and integrin-independent locomotion. We found that on
engaging the integrin-actin clutch, actin polymerization was entirely turned into
protrusion, whereas on disengagement actin underwent slippage and retrograde flow.
Remarkably, accelerated retrograde flow was balanced by an increased actin polymerization
rate; therefore, cell shape and protrusion velocity remained constant on alternating
substrates. Due to this adaptive response in polymerization dynamics, tracks of
adhesive substrate did not dictate the path of the cells. Instead, directional
guidance was exclusively provided by a soluble gradient of chemoattractant, which
endowed these 'amoeboid' cells with extraordinary flexibility, enabling them to
traverse almost every type of tissue.
acknowledgement: We thank S. Cremer for statistical analysis, K. Hirsch for technical
assistance, D. Critchley for talin1-deficient mice and R. Fässler for integrindeficient
mice, discussions and critical reading of the manuscript. This work was supported
by the German Research Foundation, the Peter Hans Hofschneider Foundation for Experimental
Biomedicine, the Max Planck Society, the Alexander von Humboldt Foundation and the
allergology programme of the Landesstiftung Baden-Württemberg.
author:
- first_name: Jörg
full_name: Renkawitz, Jörg
id: 3F0587C8-F248-11E8-B48F-1D18A9856A87
last_name: Renkawitz
orcid: 0000-0003-2856-3369
- first_name: Kathrin
full_name: Schumann, Kathrin
id: F44D762E-4F9D-11E9-B64C-9EB26CEFFB5F
last_name: Schumann
- first_name: Michele
full_name: Weber, Michele
id: 3A3FC708-F248-11E8-B48F-1D18A9856A87
last_name: Weber
- first_name: Tim
full_name: Lämmermann, Tim
last_name: Lämmermann
- first_name: Holger
full_name: Pflicke, Holger
last_name: Pflicke
- first_name: Matthieu
full_name: Piel, Matthieu
last_name: Piel
- first_name: Julien
full_name: Polleux, Julien
last_name: Polleux
- first_name: Joachim
full_name: Spatz, Joachim
last_name: Spatz
- first_name: Michael K
full_name: Sixt, Michael K
id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87
last_name: Sixt
orcid: 0000-0002-6620-9179
citation:
ama: Renkawitz J, Schumann K, Weber M, et al. Adaptive force transmission in amoeboid
cell migration. Nature Cell Biology. 2009;11(12):1438-1443. doi:10.1038/ncb1992
apa: Renkawitz, J., Schumann, K., Weber, M., Lämmermann, T., Pflicke, H., Piel,
M., … Sixt, M. K. (2009). Adaptive force transmission in amoeboid cell migration.
Nature Cell Biology. Nature Publishing Group. https://doi.org/10.1038/ncb1992
chicago: Renkawitz, Jörg, Kathrin Schumann, Michele Weber, Tim Lämmermann, Holger
Pflicke, Matthieu Piel, Julien Polleux, Joachim Spatz, and Michael K Sixt. “Adaptive
Force Transmission in Amoeboid Cell Migration.” Nature Cell Biology. Nature
Publishing Group, 2009. https://doi.org/10.1038/ncb1992.
ieee: J. Renkawitz et al., “Adaptive force transmission in amoeboid cell
migration,” Nature Cell Biology, vol. 11, no. 12. Nature Publishing Group,
pp. 1438–1443, 2009.
ista: Renkawitz J, Schumann K, Weber M, Lämmermann T, Pflicke H, Piel M, Polleux
J, Spatz J, Sixt MK. 2009. Adaptive force transmission in amoeboid cell migration.
Nature Cell Biology. 11(12), 1438–1443.
mla: Renkawitz, Jörg, et al. “Adaptive Force Transmission in Amoeboid Cell Migration.”
Nature Cell Biology, vol. 11, no. 12, Nature Publishing Group, 2009, pp.
1438–43, doi:10.1038/ncb1992.
short: J. Renkawitz, K. Schumann, M. Weber, T. Lämmermann, H. Pflicke, M. Piel,
J. Polleux, J. Spatz, M.K. Sixt, Nature Cell Biology 11 (2009) 1438–1443.
date_created: 2018-12-11T12:06:05Z
date_published: 2009-11-15T00:00:00Z
date_updated: 2021-01-12T07:53:27Z
day: '15'
doi: 10.1038/ncb1992
extern: '1'
intvolume: ' 11'
issue: '12'
language:
- iso: eng
month: '11'
oa_version: None
page: 1438 - 1443
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '2173'
status: public
title: Adaptive force transmission in amoeboid cell migration
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 11
year: '2009'
...