--- _id: '3958' abstract: - lang: eng text: Extracellular matrix (ECM) proteins can modify immune reactions, e.g. by sequestering or displaying growth factors and by interacting with immune and glial cells. Here we quantified by quantitative polymerase chain reaction (qPCR) expression of 50 ECM components and 34 ECM degrading enzymes in multiple sclerosis (MS) active and inactive white matter lesions. COL1A1, COL3A1, COL5A1 and COL5A2 chains were induced strongly in active lesions and even more in inactive lesions. These chains interact to form collagen types I, III and V, which are fibrillar collagens. Biglycan and decorin, which can decorate fibrillar collagens, were also induced strongly. The fibrillar collagens, biglycan and decorin were largely found between the endothelium and astrocytic glia limitans in the perivascular space where they formed a meshwork which was closely associated with infiltrating immune cells. In active lesions collagen V was also seen in the heavily infiltrated parenchyma. Fibrillar collagens I and III inhibited in vitro human monocyte production of CCL2 (MCP-1), an inflammatory chemokine involved in recruitment of immune cells. Together, ECM changes in lesions with different activities were quantified and proteins forming a perivascular fibrosis were identified. Induced fibrillar collagens may contribute to limiting enlargement of MS lesions by inhibiting the production of CCL2 by monocytes. author: - first_name: Hema full_name: Mohan, Hema last_name: Mohan - first_name: Markus full_name: Krumbholz, Markus last_name: Krumbholz - first_name: Rakhi full_name: Sharma, Rakhi last_name: Sharma - first_name: Sylvia full_name: Eisele, Sylvia last_name: Eisele - first_name: Andreas full_name: Junker, Andreas last_name: Junker - first_name: Michael K full_name: Michael Sixt id: 41E9FBEA-F248-11E8-B48F-1D18A9856A87 last_name: Sixt orcid: 0000-0002-6620-9179 - first_name: Jia full_name: Newcombe, Jia last_name: Newcombe - first_name: Hartmut full_name: Wekerle, Hartmut last_name: Wekerle - first_name: Reinhard full_name: Hohlfeld, Reinhard last_name: Hohlfeld - first_name: Hans full_name: Lassmann, Hans last_name: Lassmann - first_name: Edgar full_name: Meinl, Edgar last_name: Meinl citation: ama: 'Mohan H, Krumbholz M, Sharma R, et al. Extracellular matrix in multiple sclerosis lesions: fibrillar collagens, biglycan and decorin are upregulated and associated with infiltrating immune cells. Brain Pathology. 2010;20(5):966-975. doi:10.1111/j.1750-3639.2010.00399.x' apa: 'Mohan, H., Krumbholz, M., Sharma, R., Eisele, S., Junker, A., Sixt, M. K., … Meinl, E. (2010). Extracellular matrix in multiple sclerosis lesions: fibrillar collagens, biglycan and decorin are upregulated and associated with infiltrating immune cells. Brain Pathology. Wiley-Blackwell. https://doi.org/10.1111/j.1750-3639.2010.00399.x' chicago: 'Mohan, Hema, Markus Krumbholz, Rakhi Sharma, Sylvia Eisele, Andreas Junker, Michael K Sixt, Jia Newcombe, et al. “Extracellular Matrix in Multiple Sclerosis Lesions: Fibrillar Collagens, Biglycan and Decorin Are Upregulated and Associated with Infiltrating Immune Cells.” Brain Pathology. Wiley-Blackwell, 2010. https://doi.org/10.1111/j.1750-3639.2010.00399.x.' ieee: 'H. Mohan et al., “Extracellular matrix in multiple sclerosis lesions: fibrillar collagens, biglycan and decorin are upregulated and associated with infiltrating immune cells,” Brain Pathology, vol. 20, no. 5. Wiley-Blackwell, pp. 966–975, 2010.' ista: 'Mohan H, Krumbholz M, Sharma R, Eisele S, Junker A, Sixt MK, Newcombe J, Wekerle H, Hohlfeld R, Lassmann H, Meinl E. 2010. Extracellular matrix in multiple sclerosis lesions: fibrillar collagens, biglycan and decorin are upregulated and associated with infiltrating immune cells. Brain Pathology. 20(5), 966–975.' mla: 'Mohan, Hema, et al. “Extracellular Matrix in Multiple Sclerosis Lesions: Fibrillar Collagens, Biglycan and Decorin Are Upregulated and Associated with Infiltrating Immune Cells.” Brain Pathology, vol. 20, no. 5, Wiley-Blackwell, 2010, pp. 966–75, doi:10.1111/j.1750-3639.2010.00399.x.' short: H. Mohan, M. Krumbholz, R. Sharma, S. Eisele, A. Junker, M.K. Sixt, J. Newcombe, H. Wekerle, R. Hohlfeld, H. Lassmann, E. Meinl, Brain Pathology 20 (2010) 966–975. date_created: 2018-12-11T12:06:07Z date_published: 2010-09-01T00:00:00Z date_updated: 2021-01-12T07:53:28Z day: '01' doi: 10.1111/j.1750-3639.2010.00399.x extern: 1 intvolume: ' 20' issue: '5' month: '09' page: 966 - 975 publication: Brain Pathology publication_status: published publisher: Wiley-Blackwell publist_id: '2169' quality_controlled: 0 status: public title: 'Extracellular matrix in multiple sclerosis lesions: fibrillar collagens, biglycan and decorin are upregulated and associated with infiltrating immune cells' type: journal_article volume: 20 year: '2010' ...