Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice

article Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice published yes Markus Brown author 3DAB9AFC-F248-11E8-B48F-1D18A9856A87 Frank P Assen author 3A8E7F24-F248-11E8-B48F-1D18A9856A870000-0003-3470-6119 Alexander F Leithner author 3B1B77E4-F248-11E8-B48F-1D18A9856A870000-0002-1073-744X Jun Abe author Helga Schachner author Gabriele Asfour author Zsuzsanna Bagó Horváth author Jens Stein author Pavel Uhrin author Michael K Sixt author 41E9FBEA-F248-11E8-B48F-1D18A9856A870000-0002-6620-9179 Dontscho Kerjaschki author MiSi department Cytoskeletal force generation and force transduction of migrating leukocytes project Cytoskeletal force generation and force transduction of migrating leukocytes project During metastasis, malignant cells escape the primary tumor, intravasate lymphatic vessels, and reach draining sentinel lymph nodes before they colonize distant organs via the blood circulation. Although lymph node metastasis in cancer patients correlates with poor prognosis, evidence is lacking as to whether and how tumor cells enter the bloodstream via lymph nodes. To investigate this question, we delivered carcinoma cells into the lymph nodes of mice by microinfusing the cells into afferent lymphatic vessels. We found that tumor cells rapidly infiltrated the lymph node parenchyma, invaded blood vessels, and seeded lung metastases without involvement of the thoracic duct. These results suggest that the lymph node blood vessels can serve as an exit route for systemic dissemination of cancer cells in experimental mouse models. Whether this form of tumor cell spreading occurs in cancer patients remains to be determined. American Association for the Advancement of Science2018 eng Science 29567714 00042804360004710.1126/science.aal3662 35963821408 - 1411 https://research-explorer.ista.ac.at/record/6947 Brown M, Assen FP, Leithner AF, Abe J, Schachner H, Asfour G, Bagó Horváth Z, Stein J, Uhrin P, Sixt MK, Kerjaschki D. 2018. Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice. Science. 359(6382), 1408–1411. Brown, M., Assen, F. P., Leithner, A. F., Abe, J., Schachner, H., Asfour, G., … Kerjaschki, D. (2018). Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice. Science. American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.aal3662">https://doi.org/10.1126/science.aal3662</a> M. Brown, F.P. Assen, A.F. Leithner, J. Abe, H. Schachner, G. Asfour, Z. Bagó Horváth, J. Stein, P. Uhrin, M.K. Sixt, D. Kerjaschki, Science 359 (2018) 1408–1411. Brown M, Assen FP, Leithner AF, et al. Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice. Science. 2018;359(6382):1408-1411. doi:<a href="https://doi.org/10.1126/science.aal3662">10.1126/science.aal3662</a> Brown, Markus, et al. “Lymph Node Blood Vessels Provide Exit Routes for Metastatic Tumor Cell Dissemination in Mice.” Science, vol. 359, no. 6382, American Association for the Advancement of Science, 2018, pp. 1408–11, doi:<a href="https://doi.org/10.1126/science.aal3662">10.1126/science.aal3662</a>. Brown, Markus, Frank P Assen, Alexander F Leithner, Jun Abe, Helga Schachner, Gabriele Asfour, Zsuzsanna Bagó Horváth, et al. “Lymph Node Blood Vessels Provide Exit Routes for Metastatic Tumor Cell Dissemination in Mice.” Science. American Association for the Advancement of Science, 2018. <a href="https://doi.org/10.1126/science.aal3662">https://doi.org/10.1126/science.aal3662</a>. M. Brown et al., “Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice,” Science, vol. 359, no. 6382. American Association for the Advancement of Science, pp. 1408–1411, 2018. 4022018-12-11T11:46:16Z2026-06-13T22:30:55Z