---
_id: '410'
abstract:
- lang: eng
text: Lesion verification and quantification is traditionally done via histological
examination of sectioned brains, a time-consuming process that relies heavily
on manual estimation. Such methods are particularly problematic in posterior cortical
regions (e.g. visual cortex), where sectioning leads to significant damage and
distortion of tissue. Even more challenging is the post hoc localization of micro-electrodes,
which relies on the same techniques, suffers from similar drawbacks and requires
even higher precision. Here, we propose a new, simple method for quantitative
lesion characterization and electrode localization that is less labor-intensive
and yields more detailed results than conventional methods. We leverage staining
techniques standard in electron microscopy with the use of commodity micro-CT
imaging. We stain whole rat and zebra finch brains in osmium tetroxide, embed
these in resin and scan entire brains in a micro-CT machine. The scans result
in 3D reconstructions of the brains with section thickness dependent on sample
size (12–15 and 5–6 microns for rat and zebra finch respectively) that can be
segmented manually or automatically. Because the method captures the entire intact
brain volume, comparisons within and across studies are more tractable, and the
extent of lesions and electrodes may be studied with higher accuracy than with
current methods.
article_number: '5184'
article_processing_charge: No
author:
- first_name: Javier
full_name: Masís, Javier
last_name: Masís
- first_name: David
full_name: Mankus, David
last_name: Mankus
- first_name: Steffen
full_name: Wolff, Steffen
last_name: Wolff
- first_name: Grigori
full_name: Guitchounts, Grigori
last_name: Guitchounts
- first_name: Maximilian A
full_name: Jösch, Maximilian A
id: 2BD278E6-F248-11E8-B48F-1D18A9856A87
last_name: Jösch
orcid: 0000-0002-3937-1330
- first_name: David
full_name: Cox, David
last_name: Cox
citation:
ama: Masís J, Mankus D, Wolff S, Guitchounts G, Jösch MA, Cox D. A micro-CT-based
method for quantitative brain lesion characterization and electrode localization.
Scientific Reports. 2018;8(1). doi:10.1038/s41598-018-23247-z
apa: Masís, J., Mankus, D., Wolff, S., Guitchounts, G., Jösch, M. A., & Cox,
D. (2018). A micro-CT-based method for quantitative brain lesion characterization
and electrode localization. Scientific Reports. Nature Publishing Group.
https://doi.org/10.1038/s41598-018-23247-z
chicago: Masís, Javier, David Mankus, Steffen Wolff, Grigori Guitchounts, Maximilian
A Jösch, and David Cox. “A Micro-CT-Based Method for Quantitative Brain Lesion
Characterization and Electrode Localization.” Scientific Reports. Nature
Publishing Group, 2018. https://doi.org/10.1038/s41598-018-23247-z.
ieee: J. Masís, D. Mankus, S. Wolff, G. Guitchounts, M. A. Jösch, and D. Cox, “A
micro-CT-based method for quantitative brain lesion characterization and electrode
localization,” Scientific Reports, vol. 8, no. 1. Nature Publishing Group,
2018.
ista: Masís J, Mankus D, Wolff S, Guitchounts G, Jösch MA, Cox D. 2018. A micro-CT-based
method for quantitative brain lesion characterization and electrode localization.
Scientific Reports. 8(1), 5184.
mla: Masís, Javier, et al. “A Micro-CT-Based Method for Quantitative Brain Lesion
Characterization and Electrode Localization.” Scientific Reports, vol.
8, no. 1, 5184, Nature Publishing Group, 2018, doi:10.1038/s41598-018-23247-z.
short: J. Masís, D. Mankus, S. Wolff, G. Guitchounts, M.A. Jösch, D. Cox, Scientific
Reports 8 (2018).
date_created: 2018-12-11T11:46:19Z
date_published: 2018-03-26T00:00:00Z
date_updated: 2023-09-08T11:48:39Z
day: '26'
ddc:
- '571'
- '572'
department:
- _id: MaJö
doi: 10.1038/s41598-018-23247-z
external_id:
isi:
- '000428234100005'
file:
- access_level: open_access
checksum: 653fcb852f899c75b00ceee2a670d738
content_type: application/pdf
creator: system
date_created: 2018-12-12T10:10:42Z
date_updated: 2020-07-14T12:46:23Z
file_id: '4831'
file_name: IST-2018-994-v1+1_2018_Joesch_A-micro-CT-based.pdf
file_size: 2359430
relation: main_file
file_date_updated: 2020-07-14T12:46:23Z
has_accepted_license: '1'
intvolume: ' 8'
isi: 1
issue: '1'
language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '03'
oa: 1
oa_version: Published Version
publication: Scientific Reports
publication_status: published
publisher: Nature Publishing Group
publist_id: '7419'
pubrep_id: '994'
quality_controlled: '1'
scopus_import: '1'
status: public
title: A micro-CT-based method for quantitative brain lesion characterization and
electrode localization
tmp:
image: /images/cc_by.png
legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 8
year: '2018'
...