---
_id: '412'
abstract:
- lang: eng
  text: Clathrin-mediated endocytosis (CME) is a cellular trafficking process in which
    cargoes and lipids are internalized from the plasma membrane into vesicles coated
    with clathrin and adaptor proteins. CME is essential for many developmental and
    physiological processes in plants, but its underlying mechanism is not well characterised
    compared to that in yeast and animal systems. Here, we searched for new factors
    involved in CME in Arabidopsis thaliana by performing Tandem Affinity Purification
    of proteins that interact with clathrin light chain, a principal component of
    the clathrin coat. Among the confirmed interactors, we found two putative homologues
    of the clathrin-coat uncoating factor auxilin previously described in non-plant
    systems. Overexpression of AUXILIN-LIKE1 and AUXILIN-LIKE2 in A. thaliana caused
    an arrest of seedling growth and development. This was concomitant with inhibited
    endocytosis due to blocking of clathrin recruitment after the initial step of
    adaptor protein binding to the plasma membrane. By contrast, auxilin-like(1/2)
    loss-of-function lines did not present endocytosis-related developmental or cellular
    phenotypes under normal growth conditions. This work contributes to the on-going
    characterization of the endocytotic machinery in plants and provides a robust
    tool for conditionally and specifically interfering with CME in A. thaliana.
acknowledgement: We thank James Matthew Watson, Monika Borowska, and Peggy Stolt-Bergner
  at ProTech Facility of the Vienna Biocenter Core Facilities for the CRISPR/CAS9
  construct; Anna Müller for assistance with molecular cloning; Sebastian Bednarek,
  Liwen Jiang, and Daniël Van Damme for sharing published material; Matyáš Fendrych,
  Daniël Van Damme, and Lindy Abas for valuable discussions; and Martine De Cock for
  help with correcting the manuscript. This work was supported by the European Research
  Council under the European Union Seventh Framework Programme (FP7/2007-2013)/ERC
  Grant 282300 and by the Ministry of Education of the Czech Republic/MŠMT project
  NPUI-LO1417.
article_processing_charge: No
article_type: original
author:
- first_name: Maciek
  full_name: Adamowski, Maciek
  id: 45F536D2-F248-11E8-B48F-1D18A9856A87
  last_name: Adamowski
  orcid: 0000-0001-6463-5257
- first_name: Madhumitha
  full_name: Narasimhan, Madhumitha
  id: 44BF24D0-F248-11E8-B48F-1D18A9856A87
  last_name: Narasimhan
  orcid: 0000-0002-8600-0671
- first_name: Urszula
  full_name: Kania, Urszula
  id: 4AE5C486-F248-11E8-B48F-1D18A9856A87
  last_name: Kania
- first_name: Matous
  full_name: Glanc, Matous
  id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
  last_name: Glanc
  orcid: 0000-0003-0619-7783
- first_name: Geert
  full_name: De Jaeger, Geert
  last_name: De Jaeger
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. A functional
    study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors in Arabidopsis.
    <i>The Plant Cell</i>. 2018;30(3):700-716. doi:<a href="https://doi.org/10.1105/tpc.17.00785">10.1105/tpc.17.00785</a>
  apa: Adamowski, M., Narasimhan, M., Kania, U., Glanc, M., De Jaeger, G., &#38; Friml,
    J. (2018). A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating
    factors in Arabidopsis. <i>The Plant Cell</i>. American Society of Plant Biologists.
    <a href="https://doi.org/10.1105/tpc.17.00785">https://doi.org/10.1105/tpc.17.00785</a>
  chicago: Adamowski, Maciek, Madhumitha Narasimhan, Urszula Kania, Matous Glanc,
    Geert De Jaeger, and Jiří Friml. “A Functional Study of AUXILIN LIKE1 and 2 Two
    Putative Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>. American
    Society of Plant Biologists, 2018. <a href="https://doi.org/10.1105/tpc.17.00785">https://doi.org/10.1105/tpc.17.00785</a>.
  ieee: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, and J. Friml,
    “A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
    in Arabidopsis,” <i>The Plant Cell</i>, vol. 30, no. 3. American Society of Plant
    Biologists, pp. 700–716, 2018.
  ista: Adamowski M, Narasimhan M, Kania U, Glanc M, De Jaeger G, Friml J. 2018. A
    functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
    in Arabidopsis. The Plant Cell. 30(3), 700–716.
  mla: Adamowski, Maciek, et al. “A Functional Study of AUXILIN LIKE1 and 2 Two Putative
    Clathrin Uncoating Factors in Arabidopsis.” <i>The Plant Cell</i>, vol. 30, no.
    3, American Society of Plant Biologists, 2018, pp. 700–16, doi:<a href="https://doi.org/10.1105/tpc.17.00785">10.1105/tpc.17.00785</a>.
  short: M. Adamowski, M. Narasimhan, U. Kania, M. Glanc, G. De Jaeger, J. Friml,
    The Plant Cell 30 (2018) 700–716.
corr_author: '1'
date_created: 2018-12-11T11:46:20Z
date_published: 2018-04-09T00:00:00Z
date_updated: 2026-05-20T22:31:00Z
day: '09'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1105/tpc.17.00785
ec_funded: 1
external_id:
  isi:
  - '000429441400018'
  pmid:
  - '29511054'
file:
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  creator: dernst
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  date_updated: 2022-05-23T09:12:38Z
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has_accepted_license: '1'
intvolume: '        30'
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language:
- iso: eng
license: https://creativecommons.org/licenses/by/4.0/
month: '04'
oa: 1
oa_version: Published Version
page: 700 - 716
pmid: 1
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '282300'
  name: Polarity and subcellular dynamics in plants
publication: The Plant Cell
publication_identifier:
  eissn:
  - 1532-298X
  issn:
  - 1040-4651
publication_status: published
publisher: American Society of Plant Biologists
publist_id: '7417'
quality_controlled: '1'
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scopus_import: '1'
status: public
title: A functional study of AUXILIN LIKE1 and 2 two putative clathrin uncoating factors
  in Arabidopsis
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: c635000d-4b10-11ee-a964-aac5a93f6ac1
volume: 30
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...