---
_id: '4169'
abstract:
- lang: eng
  text: 'Background: During vertebrate gastrulation, cell polarization and migration
    are core components in the cellular rearrangements that lead to the formation
    of the three germ layers, ectoderm, mesoderm, and endoderm. Previous studies have
    implicated the Wnt/planar cell polarity (PCP) signaling pathway in controlling
    cell morphology and movement during gastrulation. However, cell polarization and
    directed cell migration are reduced but not completely abolished in the absence
    of Wnt/PCP signals; this observation indicates that other signaling pathways must
    be involved. Results: We show that Phosphoinositide 3-Kinases (PI3Ks) are required
    at the onset of zebrafish gastrulation in mesendodermal cells for process formation
    and cell polarization. Platelet Derived Growth Factor (PDGF) functions upstream
    of PI3K, while Protein Kinase B (PKB), a downstream effector of PI3K activity,
    localizes to the leading edge of migrating mesendodermal cells. In the absence
    of PI3K activity, PKB localization and cell polarization are strongly reduced
    in mesendodermal cells and are followed by slower but still highly coordinated
    and directed movements of these cells. Conclusions: We have identified a novel
    role of a signaling pathway comprised of PDGF, PI3K, and PKB in the control of
    morphogenetic cell movements during gastrulation. Furthermore, our findings provide
    insight into the relationship between cell polarization and directed cell migration
    at the onset of zebrafish gastrulation.'
acknowledgement: 'We would like to thank Jennifer Geiger, Juan Hurl& Hannu Mansu-koski,
  Florian Raible, Marino Zerial, Steve Wilson, and Kurt Anderson for critical reading
  of earlier versions of this manuscript. We thank Erez Raz, Bart Vanhaesebroeck,
  and Lukas Roth for sending us the pCS2-PH-GFP-nos, the p1IOCAAX, and the pCS2-actin-GFP
  constructs, respectively. We are grateful to Marino Zerial and his lab for encouraging
  us to start this work and providing us with the dnP13K construct and to Florian
  Ulrich and Franziska Friedrich for help with the confocal microscope and artwork,
  respectively. We thank Gunter Junghanns and Evelyn Lehmann for excellent fish care.
  C.-P.H. is supported by an Emmy-Noother-Fellowship from the Deutsche Forschungsgemeinschaft. '
article_processing_charge: No
article_type: original
author:
- first_name: Juan
  full_name: Montero, Juan
  last_name: Montero
- first_name: Beate
  full_name: Kilian, Beate
  last_name: Kilian
- first_name: Joanne
  full_name: Chan, Joanne
  last_name: Chan
- first_name: Peter
  full_name: Bayliss, Peter
  last_name: Bayliss
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
citation:
  ama: Montero J, Kilian B, Chan J, Bayliss P, Heisenberg C-PJ. Phosphoinositide 3-kinase
    is required for process outgrowth and cell polarization of gastrulating mesendodermal
    cells. <i>Current Biology</i>. 2003;13(15):1279-1289. doi:<a href="https://doi.org/10.1016/S0960-9822(03)00505-0">10.1016/S0960-9822(03)00505-0</a>
  apa: Montero, J., Kilian, B., Chan, J., Bayliss, P., &#38; Heisenberg, C.-P. J.
    (2003). Phosphoinositide 3-kinase is required for process outgrowth and cell polarization
    of gastrulating mesendodermal cells. <i>Current Biology</i>. Cell Press. <a href="https://doi.org/10.1016/S0960-9822(03)00505-0">https://doi.org/10.1016/S0960-9822(03)00505-0</a>
  chicago: Montero, Juan, Beate Kilian, Joanne Chan, Peter Bayliss, and Carl-Philipp
    J Heisenberg. “Phosphoinositide 3-Kinase Is Required for Process Outgrowth and
    Cell Polarization of Gastrulating Mesendodermal Cells.” <i>Current Biology</i>.
    Cell Press, 2003. <a href="https://doi.org/10.1016/S0960-9822(03)00505-0">https://doi.org/10.1016/S0960-9822(03)00505-0</a>.
  ieee: J. Montero, B. Kilian, J. Chan, P. Bayliss, and C.-P. J. Heisenberg, “Phosphoinositide
    3-kinase is required for process outgrowth and cell polarization of gastrulating
    mesendodermal cells,” <i>Current Biology</i>, vol. 13, no. 15. Cell Press, pp.
    1279–1289, 2003.
  ista: Montero J, Kilian B, Chan J, Bayliss P, Heisenberg C-PJ. 2003. Phosphoinositide
    3-kinase is required for process outgrowth and cell polarization of gastrulating
    mesendodermal cells. Current Biology. 13(15), 1279–1289.
  mla: Montero, Juan, et al. “Phosphoinositide 3-Kinase Is Required for Process Outgrowth
    and Cell Polarization of Gastrulating Mesendodermal Cells.” <i>Current Biology</i>,
    vol. 13, no. 15, Cell Press, 2003, pp. 1279–89, doi:<a href="https://doi.org/10.1016/S0960-9822(03)00505-0">10.1016/S0960-9822(03)00505-0</a>.
  short: J. Montero, B. Kilian, J. Chan, P. Bayliss, C.-P.J. Heisenberg, Current Biology
    13 (2003) 1279–1289.
date_created: 2018-12-11T12:07:22Z
date_published: 2003-08-05T00:00:00Z
date_updated: 2024-02-27T10:03:37Z
day: '05'
doi: 10.1016/S0960-9822(03)00505-0
extern: '1'
external_id:
  pmid:
  - ' 12906787'
intvolume: '        13'
issue: '15'
language:
- iso: eng
month: '08'
oa_version: None
page: 1279 - 1289
pmid: 1
publication: Current Biology
publication_identifier:
  eissn:
  - 1879-0445
  issn:
  - 0960-9822
publication_status: published
publisher: Cell Press
publist_id: '1950'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Phosphoinositide 3-kinase is required for process outgrowth and cell polarization
  of gastrulating mesendodermal cells
type: journal_article
user_id: ea97e931-d5af-11eb-85d4-e6957dddbf17
volume: 13
year: '2003'
...