---
_id: '4187'
abstract:
- lang: eng
text: Cell migration is central to embryonic development, homeostasis and disease(1),
processes in which cells move as part of a group or individually. Whereas the
mechanisms controlling single-cell migration in vitro are relatively well understood(2-4),
less is known about the mechanisms promoting the motility of individual cells
in vivo. In particular, it is not clear how cells that form blebs in their migration
use those protrusions to bring about movement in the context of the three-dimensional
cellular environment(5,6). Here we show that the motility of chemokine-guided
germ cells within the zebrafish embryo requires the function of the small Rho
GTPases Rac1 and RhoA, as well as E-cadherin-mediated cell-cell adhesion. Using
fluorescence resonance energy transfer we demonstrate that Rac1 and RhoA are activated
in the cell front. At this location, Rac1 is responsible for the formation of
actin-rich structures, and RhoA promotes retrograde actin flow. We propose that
these actin-rich structures undergoing retrograde flow are essential for the generation
of E-cadherin-mediated traction forces between the germ cells and the surrounding
tissue and are therefore crucial for cell motility in vivo.
author:
- first_name: Elena
full_name: Kardash, Elena
last_name: Kardash
- first_name: Michal
full_name: Reichman-Fried, Michal
last_name: Reichman Fried
- first_name: Jean
full_name: Maître, Jean-Léon
last_name: Maître
- first_name: Bijan
full_name: Boldajipour, Bijan
last_name: Boldajipour
- first_name: Ekaterina
full_name: Ekaterina Papusheva
id: 41DB591E-F248-11E8-B48F-1D18A9856A87
last_name: Papusheva
- first_name: Esther
full_name: Messerschmidt, Esther-Maria
last_name: Messerschmidt
- first_name: Carl
full_name: Heisenberg, Carl-Philipp
last_name: Heisenberg
- first_name: Erez
full_name: Raz, Erez
last_name: Raz
citation:
ama: Kardash E, Reichman Fried M, Maître J, et al. A role for Rho GTPases and cell-cell
adhesion in single-cell motility in vivo. Nature Cell Biology. 2010;12(1):47-53.
doi:10.1038/ncb2003
apa: Kardash, E., Reichman Fried, M., Maître, J., Boldajipour, B., Papusheva, E.,
Messerschmidt, E., … Raz, E. (2010). A role for Rho GTPases and cell-cell adhesion
in single-cell motility in vivo. Nature Cell Biology. Nature Publishing
Group. https://doi.org/10.1038/ncb2003
chicago: Kardash, Elena, Michal Reichman Fried, Jean Maître, Bijan Boldajipour,
Ekaterina Papusheva, Esther Messerschmidt, Carl Heisenberg, and Erez Raz. “A Role
for Rho GTPases and Cell-Cell Adhesion in Single-Cell Motility in Vivo.” Nature
Cell Biology. Nature Publishing Group, 2010. https://doi.org/10.1038/ncb2003.
ieee: E. Kardash et al., “A role for Rho GTPases and cell-cell adhesion in
single-cell motility in vivo,” Nature Cell Biology, vol. 12, no. 1. Nature
Publishing Group, pp. 47–53, 2010.
ista: Kardash E, Reichman Fried M, Maître J, Boldajipour B, Papusheva E, Messerschmidt
E, Heisenberg C, Raz E. 2010. A role for Rho GTPases and cell-cell adhesion in
single-cell motility in vivo. Nature Cell Biology. 12(1), 47–53.
mla: Kardash, Elena, et al. “A Role for Rho GTPases and Cell-Cell Adhesion in Single-Cell
Motility in Vivo.” Nature Cell Biology, vol. 12, no. 1, Nature Publishing
Group, 2010, pp. 47–53, doi:10.1038/ncb2003.
short: E. Kardash, M. Reichman Fried, J. Maître, B. Boldajipour, E. Papusheva, E.
Messerschmidt, C. Heisenberg, E. Raz, Nature Cell Biology 12 (2010) 47–53.
date_created: 2018-12-11T12:07:28Z
date_published: 2010-01-01T00:00:00Z
date_updated: 2021-01-12T07:55:09Z
day: '01'
doi: 10.1038/ncb2003
extern: 1
fulldoi: https://doi.org/10.1038/ncb2003
intvolume: ' 12'
issue: '1'
month: '01'
page: 47 - 53
publication: Nature Cell Biology
publication_status: published
publisher: Nature Publishing Group
publist_id: '1932'
quality_controlled: 0
status: public
title: A role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo
type: journal_article
volume: 12
year: '2010'
...