--- res: bibo_abstract: - 'Mutations in two genes affect the formation of the boundary between midbrain and hindbrain (MHB): no isthmus (noi) and acerebellar (ace), noi mutant embryos lack the MHB constriction, the cerebellum and optic tectum, as well as the pronephric duct. Analysis of noi mutant embryos with neuron-specific antibodies shows that the MHB region and the dorsal and ventral midbrain are absent or abnormal, but that the rostral hindbrain is unaffected with the exception of the cerebellum, Using markers that are expressed during its formation (eng, wnt1 and pax-b), we find that the MHB region is already misspecified in noi mutant embryos during late gastrulation. The tectum is initially present and later degenerates, The defect in ace mutant embryos is more restricted: MHB and cerebellum are absent, but a tectum is formed, Molecular organisation of the tectum and tegmentum is disturbed, however, since eng, wntl and pax-b marker gene expression is not maintained, We propose that noi and ace are required for development of the MHB region and of the adjacent mid- and hindbrain, which are thought to be patterned by the MHB region, Presence of pax-b RNA, and absence of pax-b protein, together with the observation of genetic linkage and the occurrence of a point mutation, show that noi mutations are located in the pax-b gene, pax-b is a vertebrate orthologue of the Drosophila gene paired, which is involved in a pathway of cellular interactions at the posterior compartment boundary in Drosophila, Our results confirm and extend a previous report, and show that at least one member of this conserved signalling pathway is required for formation of the boundary between midbrain and hindbrain in the zebrafish.@eng' bibo_authorlist: - foaf_Person: foaf_givenName: Michael foaf_name: Brand, Michael foaf_surname: Brand - foaf_Person: foaf_givenName: Carl-Philipp J foaf_name: Heisenberg, Carl-Philipp J foaf_surname: Heisenberg foaf_workInfoHomepage: http://www.librecat.org/personId=39427864-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-0912-4566 - foaf_Person: foaf_givenName: Yunjin foaf_name: Jiang, Yunjin foaf_surname: Jiang - foaf_Person: foaf_givenName: Dirk foaf_name: Beuchle, Dirk foaf_surname: Beuchle - foaf_Person: foaf_givenName: Klaus foaf_name: Lun, Klaus foaf_surname: Lun - foaf_Person: foaf_givenName: Makoto foaf_name: Furutani Seiki, Makoto foaf_surname: Furutani Seiki - foaf_Person: foaf_givenName: Michael foaf_name: Granato, Michael foaf_surname: Granato - foaf_Person: foaf_givenName: Pascal foaf_name: Haffter, Pascal foaf_surname: Haffter - foaf_Person: foaf_givenName: Matthias foaf_name: Hammerschmidt, Matthias foaf_surname: Hammerschmidt - foaf_Person: foaf_givenName: Donald foaf_name: Kane, Donald foaf_surname: Kane - foaf_Person: foaf_givenName: Robert foaf_name: Kelsh, Robert foaf_surname: Kelsh - foaf_Person: foaf_givenName: Mary foaf_name: Mullins, Mary foaf_surname: Mullins - foaf_Person: foaf_givenName: Jörg foaf_name: Odenthal, Jörg foaf_surname: Odenthal - foaf_Person: foaf_givenName: Fredericus foaf_name: Van Eeden, Fredericus foaf_surname: Van Eeden - foaf_Person: foaf_givenName: Christiane foaf_name: Nüsslein Volhard, Christiane foaf_surname: Nüsslein Volhard bibo_doi: '10.1242/dev.123.1.179 ' bibo_issue: '1' bibo_volume: 123 dct_date: 1996^xs_gYear dct_isPartOf: - http://id.crossref.org/issn/0950-1991 dct_language: eng dct_publisher: Company of Biologists@ dct_title: Mutations in zebrafish genes affecting the formation of the boundary between midbrain and hindbrain@ fabio_hasPubmedId: '9007239 ' ...