Kinetics of morphogen gradient formation Anna Kicheva ; https://orcid.org/0000-0003-4509-4998 Pantazis, Periklis Bollenbach, Tobias Kalaidzidis, Yannis Bittig, Thomas Julicher, Frank Gonzalez-Gaitan, Marcos In the developing fly wing, secreted morphogens such as Decapentaplegic (Dpp) and Wingless (Wg) form gradients of concentration providing positional information. Dpp forms a longer-range gradient than Wg. To understand how the range is controlled, we measured the four key kinetic parameters governing morphogen spreading: the production rate, the effective diffusion coefficient, the degradation rate, and the immobile fraction. The four parameters had different values for Dpp versus Wg. In addition, Dynamin-dependent endocytosis was required for spreading of Dpp, but not Wg. Thus, the cellular mechanisms of Dpp and Wingless spreading are different: Dpp spreading requires endocytic, intracellular trafficking. American Association for the Advancement of Science 2007 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/4226 Kicheva A, Pantazis P, Bollenbach T, et al. Kinetics of morphogen gradient formation. <i>Science</i>. 2007;315(5811):521-525. doi:<a href="https://doi.org/10.1126/science.1135774">10.1126/science.1135774</a> info:eu-repo/semantics/altIdentifier/doi/10.1126/science.1135774 info:eu-repo/semantics/closedAccess