---
_id: '428'
abstract:
- lang: eng
text: The plant hormone gibberellic acid (GA) is a crucial regulator of growth and
development. The main paradigm of GA signaling puts forward transcriptional regulation
via the degradation of DELLA transcriptional repressors. GA has also been shown
to regulate tropic responses by modulation of the plasma membrane incidence of
PIN auxin transporters by an unclear mechanism. Here we uncovered the cellular
and molecular mechanisms by which GA redirects protein trafficking and thus regulates
cell surface functionality. Photoconvertible reporters revealed that GA balances
the protein traffic between the vacuole degradation route and recycling back to
the cell surface. Low GA levels promote vacuolar delivery and degradation of multiple
cargos, including PIN proteins, whereas high GA levels promote their recycling
to the plasma membrane. This GA effect requires components of the retromer complex,
such as Sorting Nexin 1 (SNX1) and its interacting, microtubule (MT)-associated
protein, the Cytoplasmic Linker-Associated Protein (CLASP1). Accordingly, GA regulates
the subcellular distribution of SNX1 and CLASP1, and the intact MT cytoskeleton
is essential for the GA effect on trafficking. This GA cellular action occurs
through DELLA proteins that regulate the MT and retromer presumably via their
interaction partners Prefoldins (PFDs). Our study identified a branching of the
GA signaling pathway at the level of DELLA proteins, which, in parallel to regulating
transcription, also target by a nontranscriptional mechanism the retromer complex
acting at the intersection of the degradation and recycling trafficking routes.
By this mechanism, GA can redirect receptors and transporters to the cell surface,
thus coregulating multiple processes, including PIN-dependent auxin fluxes during
tropic responses.
acknowledgement: "We gratefully acknowledge M. Blázquez (Instituto de Biología Molecular
y Celular de Plantas), M. Fendrych, C. Cuesta Moliner (Institute of Science and
Technology Austria), M. Vanstraelen, M. Nowack (Center for Plant Systems Biology,
Ghent), C. Luschnig (Universitat fur Bodenkultur Wien, Vienna), S. Simon (Central
European Institute of Technology, Brno), C. Sommerville (Carnegie Institution for
Science), and Y. Gu (Penn State University) for making available the materials used
in this study;\r\n...funding from the European Research Council (ERC) under the
European Union’s Seventh Framework Programme (FP7/2007-2013)/ERC Grant Agreement
282300.\r\nCC BY NC ND"
article_processing_charge: No
author:
- first_name: Yuliya
full_name: Salanenka, Yuliya
id: 46DAAE7E-F248-11E8-B48F-1D18A9856A87
last_name: Salanenka
- first_name: Inge
full_name: Verstraeten, Inge
id: 362BF7FE-F248-11E8-B48F-1D18A9856A87
last_name: Verstraeten
orcid: 0000-0001-7241-2328
- first_name: Christian
full_name: Löfke, Christian
last_name: Löfke
- first_name: Kaori
full_name: Tabata, Kaori
id: 7DAAEDA4-02D0-11E9-B11A-A5A4D7DFFFD0
last_name: Tabata
- first_name: Satoshi
full_name: Naramoto, Satoshi
last_name: Naramoto
- first_name: Matous
full_name: Glanc, Matous
id: 1AE1EA24-02D0-11E9-9BAA-DAF4881429F2
last_name: Glanc
orcid: 0000-0003-0619-7783
- first_name: Jirí
full_name: Friml, Jirí
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
citation:
ama: Salanenka Y, Verstraeten I, Löfke C, et al. Gibberellin DELLA signaling targets
the retromer complex to redirect protein trafficking to the plasma membrane. Proceedings
of the National Academy of Sciences of the United States of America. 2018;115(14):3716-3721.
doi:10.1073/pnas.1721760115
apa: Salanenka, Y., Verstraeten, I., Löfke, C., Tabata, K., Naramoto, S., Glanc,
M., & Friml, J. (2018). Gibberellin DELLA signaling targets the retromer complex
to redirect protein trafficking to the plasma membrane. Proceedings of the
National Academy of Sciences of the United States of America. National Academy
of Sciences. https://doi.org/10.1073/pnas.1721760115
chicago: Salanenka, Yuliya, Inge Verstraeten, Christian Löfke, Kaori Tabata, Satoshi
Naramoto, Matous Glanc, and Jiří Friml. “Gibberellin DELLA Signaling Targets the
Retromer Complex to Redirect Protein Trafficking to the Plasma Membrane.” Proceedings
of the National Academy of Sciences of the United States of America. National
Academy of Sciences, 2018. https://doi.org/10.1073/pnas.1721760115.
ieee: Y. Salanenka et al., “Gibberellin DELLA signaling targets the retromer
complex to redirect protein trafficking to the plasma membrane,” Proceedings
of the National Academy of Sciences of the United States of America, vol.
115, no. 14. National Academy of Sciences, pp. 3716–3721, 2018.
ista: Salanenka Y, Verstraeten I, Löfke C, Tabata K, Naramoto S, Glanc M, Friml
J. 2018. Gibberellin DELLA signaling targets the retromer complex to redirect
protein trafficking to the plasma membrane. Proceedings of the National Academy
of Sciences of the United States of America. 115(14), 3716–3721.
mla: Salanenka, Yuliya, et al. “Gibberellin DELLA Signaling Targets the Retromer
Complex to Redirect Protein Trafficking to the Plasma Membrane.” Proceedings
of the National Academy of Sciences of the United States of America, vol.
115, no. 14, National Academy of Sciences, 2018, pp. 3716–21, doi:10.1073/pnas.1721760115.
short: Y. Salanenka, I. Verstraeten, C. Löfke, K. Tabata, S. Naramoto, M. Glanc,
J. Friml, Proceedings of the National Academy of Sciences of the United States
of America 115 (2018) 3716–3721.
corr_author: '1'
date_created: 2018-12-11T11:46:25Z
date_published: 2018-04-03T00:00:00Z
date_updated: 2025-06-03T11:21:29Z
day: '03'
ddc:
- '580'
department:
- _id: JiFr
doi: 10.1073/pnas.1721760115
ec_funded: 1
external_id:
isi:
- '000429012500073'
file:
- access_level: open_access
checksum: 1fcf7223fb8f99559cfa80bd6f24ce44
content_type: application/pdf
creator: dernst
date_created: 2018-12-17T12:30:14Z
date_updated: 2020-07-14T12:46:26Z
file_id: '5700'
file_name: 2018_PNAS_Salanenka.pdf
file_size: 1924101
relation: main_file
file_date_updated: 2020-07-14T12:46:26Z
fulldoi: https://doi.org/10.1073/pnas.1721760115
has_accepted_license: '1'
intvolume: ' 115'
isi: 1
issue: '14'
language:
- iso: eng
license: https://creativecommons.org/licenses/by-nc-nd/4.0/
month: '04'
oa: 1
oa_version: Published Version
page: ' 3716 - 3721'
project:
- _id: 25716A02-B435-11E9-9278-68D0E5697425
call_identifier: FP7
grant_number: '282300'
name: Polarity and subcellular dynamics in plants
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_status: published
publisher: National Academy of Sciences
publist_id: '7395'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Gibberellin DELLA signaling targets the retromer complex to redirect protein
trafficking to the plasma membrane
tmp:
image: /images/cc_by_nc_nd.png
legal_code_url: https://creativecommons.org/licenses/by-nc-nd/4.0/legalcode
name: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
(CC BY-NC-ND 4.0)
short: CC BY-NC-ND (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 115
year: '2018'
...