{"page":"2918-2931","publication_status":"published","date_published":"2018-04-06T00:00:00Z","publisher":"Oxford University Press","type":"journal_article","intvolume":"        46","pubrep_id":"971","abstract":[{"lang":"eng","text":"The MazF toxin sequence-specifically cleaves single-stranded RNA upon various stressful conditions, and it is activated as a part of the mazEF toxin–antitoxin module in Escherichia coli. Although autoregulation of mazEF expression through the MazE antitoxin-dependent transcriptional repression has been biochemically characterized, less is known about post-transcriptional autoregulation, as well as how both of these autoregulatory features affect growth of single cells during conditions that promote MazF production. Here, we demonstrate post-transcriptional autoregulation of mazF expression dynamics by MazF cleaving its own transcript. Single-cell analyses of bacterial populations during ectopic MazF production indicated that two-level autoregulation of mazEF expression influences cell-to-cell growth rate heterogeneity. The increase in growth rate heterogeneity is governed by the MazE antitoxin, and tuned by the MazF-dependent mazF mRNA cleavage. Also, both autoregulatory features grant rapid exit from the stress caused by mazF overexpression. Time-lapse microscopy revealed that MazF-mediated cleavage of mazF mRNA leads to increased temporal variability in length of individual cells during ectopic mazF overexpression, as explained by a stochastic model indicating that mazEF mRNA cleavage underlies temporal fluctuations in MazF levels during stress."}],"article_processing_charge":"Yes (in subscription journal)","file_date_updated":"2020-07-14T12:46:27Z","status":"public","title":"Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations","issue":"6","user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","month":"04","license":"https://creativecommons.org/licenses/by/4.0/","external_id":{"isi":["000429009500021"]},"publication":"Nucleic Acids Research","language":[{"iso":"eng"}],"related_material":{"record":[{"relation":"popular_science","status":"public","id":"5569"}]},"oa_version":"Published Version","date_created":"2018-12-11T11:46:29Z","project":[{"name":"FWF Open Access Fund","call_identifier":"FWF","_id":"3AC91DDA-15DF-11EA-824D-93A3E7B544D1"}],"scopus_import":"1","_id":"438","year":"2018","isi":1,"has_accepted_license":"1","file":[{"relation":"main_file","checksum":"3ff4f545c27e11a4cd20ccb30778793e","date_created":"2018-12-12T10:15:30Z","file_id":"5151","content_type":"application/pdf","file_name":"IST-2018-971-v1+1_2018_Nikoloc_Autoregulation_of.pdf","file_size":5027978,"access_level":"open_access","creator":"system","date_updated":"2020-07-14T12:46:27Z"}],"author":[{"id":"42D9CABC-F248-11E8-B48F-1D18A9856A87","first_name":"Nela","full_name":"Nikolic, Nela","last_name":"Nikolic","orcid":"0000-0001-9068-6090"},{"full_name":"Bergmiller, Tobias","orcid":"0000-0001-5396-4346","last_name":"Bergmiller","first_name":"Tobias","id":"2C471CFA-F248-11E8-B48F-1D18A9856A87"},{"first_name":"Alexandra","last_name":"Vandervelde","full_name":"Vandervelde, Alexandra"},{"full_name":"Albanese, Tanino","last_name":"Albanese","first_name":"Tanino"},{"last_name":"Gelens","full_name":"Gelens, Lendert","first_name":"Lendert"},{"first_name":"Isabella","full_name":"Moll, Isabella","last_name":"Moll"}],"department":[{"_id":"CaGu"}],"tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"date_updated":"2024-02-21T13:44:45Z","doi":"10.1093/nar/gky079","day":"06","quality_controlled":"1","citation":{"chicago":"Nikolic, Nela, Tobias Bergmiller, Alexandra Vandervelde, Tanino Albanese, Lendert Gelens, and Isabella Moll. “Autoregulation of MazEF Expression Underlies Growth Heterogeneity in Bacterial Populations.” <i>Nucleic Acids Research</i>. Oxford University Press, 2018. <a href=\"https://doi.org/10.1093/nar/gky079\">https://doi.org/10.1093/nar/gky079</a>.","mla":"Nikolic, Nela, et al. “Autoregulation of MazEF Expression Underlies Growth Heterogeneity in Bacterial Populations.” <i>Nucleic Acids Research</i>, vol. 46, no. 6, Oxford University Press, 2018, pp. 2918–31, doi:<a href=\"https://doi.org/10.1093/nar/gky079\">10.1093/nar/gky079</a>.","ista":"Nikolic N, Bergmiller T, Vandervelde A, Albanese T, Gelens L, Moll I. 2018. Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations. Nucleic Acids Research. 46(6), 2918–2931.","short":"N. Nikolic, T. Bergmiller, A. Vandervelde, T. Albanese, L. Gelens, I. Moll, Nucleic Acids Research 46 (2018) 2918–2931.","ieee":"N. Nikolic, T. Bergmiller, A. Vandervelde, T. Albanese, L. Gelens, and I. Moll, “Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations,” <i>Nucleic Acids Research</i>, vol. 46, no. 6. Oxford University Press, pp. 2918–2931, 2018.","ama":"Nikolic N, Bergmiller T, Vandervelde A, Albanese T, Gelens L, Moll I. Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations. <i>Nucleic Acids Research</i>. 2018;46(6):2918-2931. doi:<a href=\"https://doi.org/10.1093/nar/gky079\">10.1093/nar/gky079</a>","apa":"Nikolic, N., Bergmiller, T., Vandervelde, A., Albanese, T., Gelens, L., &#38; Moll, I. (2018). Autoregulation of mazEF expression underlies growth heterogeneity in bacterial populations. <i>Nucleic Acids Research</i>. Oxford University Press. <a href=\"https://doi.org/10.1093/nar/gky079\">https://doi.org/10.1093/nar/gky079</a>"},"oa":1,"ddc":["576"],"volume":46}