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<titleInfo><title>Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls</title></titleInfo>


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<name type="personal">
  <namePart type="given">Lanxin</namePart>
  <namePart type="family">Li</namePart>
  <role><roleTerm type="text">author</roleTerm> </role><identifier type="local">367EF8FA-F248-11E8-B48F-1D18A9856A87</identifier><description xsi:type="identifierDefinition" type="orcid">0000-0002-5607-272X</description></name>
<name type="personal">
  <namePart type="given">Gabriel</namePart>
  <namePart type="family">Krens</namePart>
  <role><roleTerm type="text">author</roleTerm> </role><identifier type="local">2B819732-F248-11E8-B48F-1D18A9856A87</identifier><description xsi:type="identifierDefinition" type="orcid">0000-0003-4761-5996</description></name>
<name type="personal">
  <namePart type="given">Matyas</namePart>
  <namePart type="family">Fendrych</namePart>
  <role><roleTerm type="text">author</roleTerm> </role><identifier type="local">43905548-F248-11E8-B48F-1D18A9856A87</identifier><description xsi:type="identifierDefinition" type="orcid">0000-0002-9767-8699</description></name>
<name type="personal">
  <namePart type="given">Jirí</namePart>
  <namePart type="family">Friml</namePart>
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<abstract lang="eng">The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH.</abstract>

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<originInfo><publisher>Bio-protocol</publisher><dateIssued encoding="w3cdtf">2018</dateIssued>
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  <identifier type="eIssn">2331-8325</identifier><identifier type="doi">10.21769/BioProtoc.2685</identifier>
<part><detail type="volume"><number>8</number></detail><detail type="issue"><number>1</number></detail>
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<chicago>Li, Lanxin, Gabriel Krens, Matyas Fendrych, and Jiří Friml. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” &lt;i&gt;Bio-Protocol&lt;/i&gt;. Bio-protocol, 2018. &lt;a href=&quot;https://doi.org/10.21769/BioProtoc.2685&quot;&gt;https://doi.org/10.21769/BioProtoc.2685&lt;/a&gt;.</chicago>
<ieee>L. Li, G. Krens, M. Fendrych, and J. Friml, “Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls,” &lt;i&gt;Bio-protocol&lt;/i&gt;, vol. 8, no. 1. Bio-protocol, 2018.</ieee>
<mla>Li, Lanxin, et al. “Real-Time Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana Hypocotyls.” &lt;i&gt;Bio-Protocol&lt;/i&gt;, vol. 8, no. 1, Bio-protocol, 2018, doi:&lt;a href=&quot;https://doi.org/10.21769/BioProtoc.2685&quot;&gt;10.21769/BioProtoc.2685&lt;/a&gt;.</mla>
<ista>Li L, Krens G, Fendrych M, Friml J. 2018. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol. 8(1).</ista>
<apa>Li, L., Krens, G., Fendrych, M., &amp;#38; Friml, J. (2018). Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. &lt;i&gt;Bio-Protocol&lt;/i&gt;. Bio-protocol. &lt;a href=&quot;https://doi.org/10.21769/BioProtoc.2685&quot;&gt;https://doi.org/10.21769/BioProtoc.2685&lt;/a&gt;</apa>
<ama>Li L, Krens G, Fendrych M, Friml J. Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. &lt;i&gt;Bio-protocol&lt;/i&gt;. 2018;8(1). doi:&lt;a href=&quot;https://doi.org/10.21769/BioProtoc.2685&quot;&gt;10.21769/BioProtoc.2685&lt;/a&gt;</ama>
<short>L. Li, G. Krens, M. Fendrych, J. Friml, Bio-Protocol 8 (2018).</short>
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