---
_id: '442'
abstract:
- lang: eng
  text: The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the
    nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the
    apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the
    method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin
    response in hypocotyl segments as well as the determination of relative values
    of the cell wall pH.
acknowledgement: 'This protocol was adapted from Fendrych et al., 2016. This project
  has received funding from the European Union’s Horizon 2020 research and innovation
  programme under the Marie Skłodowska-Curie Grant Agreement No. 665385, and Austrian
  Science Fund (FWF) [M 2128-B21]. '
article_processing_charge: No
article_type: original
author:
- first_name: Lanxin
  full_name: Li, Lanxin
  id: 367EF8FA-F248-11E8-B48F-1D18A9856A87
  last_name: Li
  orcid: 0000-0002-5607-272X
- first_name: Gabriel
  full_name: Krens, Gabriel
  id: 2B819732-F248-11E8-B48F-1D18A9856A87
  last_name: Krens
  orcid: 0000-0003-4761-5996
- first_name: Matyas
  full_name: Fendrych, Matyas
  id: 43905548-F248-11E8-B48F-1D18A9856A87
  last_name: Fendrych
  orcid: 0000-0002-9767-8699
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: Li L, Krens G, Fendrych M, Friml J. Real-time analysis of auxin response, cell
    wall pH and elongation in Arabidopsis thaliana Hypocotyls. <i>Bio-protocol</i>.
    2018;8(1). doi:<a href="https://doi.org/10.21769/BioProtoc.2685">10.21769/BioProtoc.2685</a>
  apa: Li, L., Krens, G., Fendrych, M., &#38; Friml, J. (2018). Real-time analysis
    of auxin response, cell wall pH and elongation in Arabidopsis thaliana Hypocotyls.
    <i>Bio-Protocol</i>. Bio-protocol. <a href="https://doi.org/10.21769/BioProtoc.2685">https://doi.org/10.21769/BioProtoc.2685</a>
  chicago: Li, Lanxin, Gabriel Krens, Matyas Fendrych, and Jiří Friml. “Real-Time
    Analysis of Auxin Response, Cell Wall PH and Elongation in Arabidopsis Thaliana
    Hypocotyls.” <i>Bio-Protocol</i>. Bio-protocol, 2018. <a href="https://doi.org/10.21769/BioProtoc.2685">https://doi.org/10.21769/BioProtoc.2685</a>.
  ieee: L. Li, G. Krens, M. Fendrych, and J. Friml, “Real-time analysis of auxin response,
    cell wall pH and elongation in Arabidopsis thaliana Hypocotyls,” <i>Bio-protocol</i>,
    vol. 8, no. 1. Bio-protocol, 2018.
  ista: Li L, Krens G, Fendrych M, Friml J. 2018. Real-time analysis of auxin response,
    cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol.
    8(1).
  mla: Li, Lanxin, et al. “Real-Time Analysis of Auxin Response, Cell Wall PH and
    Elongation in Arabidopsis Thaliana Hypocotyls.” <i>Bio-Protocol</i>, vol. 8, no.
    1, Bio-protocol, 2018, doi:<a href="https://doi.org/10.21769/BioProtoc.2685">10.21769/BioProtoc.2685</a>.
  short: L. Li, G. Krens, M. Fendrych, J. Friml, Bio-Protocol 8 (2018).
corr_author: '1'
date_created: 2018-12-11T11:46:30Z
date_published: 2018-01-05T00:00:00Z
date_updated: 2026-03-16T23:30:44Z
day: '05'
ddc:
- '576'
- '581'
department:
- _id: JiFr
- _id: Bio
doi: 10.21769/BioProtoc.2685
ec_funded: 1
file:
- access_level: open_access
  checksum: 6644ba698206eda32b0abf09128e63e3
  content_type: application/pdf
  creator: system
  date_created: 2018-12-12T10:17:43Z
  date_updated: 2020-07-14T12:46:29Z
  file_id: '5299'
  file_name: IST-2018-970-v1+1_2018_Lanxin_Real-time_analysis.pdf
  file_size: 11352389
  relation: main_file
file_date_updated: 2020-07-14T12:46:29Z
has_accepted_license: '1'
intvolume: '         8'
issue: '1'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Published Version
project:
- _id: 2564DBCA-B435-11E9-9278-68D0E5697425
  call_identifier: H2020
  grant_number: '665385'
  name: International IST Doctoral Program
publication: Bio-protocol
publication_identifier:
  eissn:
  - 2331-8325
publication_status: published
publisher: Bio-protocol
publist_id: '7381'
pubrep_id: '970'
quality_controlled: '1'
related_material:
  record:
  - id: '10083'
    relation: dissertation_contains
    status: public
status: public
title: Real-time analysis of auxin response, cell wall pH and elongation in Arabidopsis
  thaliana Hypocotyls
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 8
year: '2018'
...