--- res: bibo_abstract: - Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain- and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Tomas foaf_name: Prat, Tomas foaf_surname: Prat foaf_workInfoHomepage: http://www.librecat.org/personId=3DA3BFEE-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Jakub foaf_name: Hajny, Jakub foaf_surname: Hajny foaf_workInfoHomepage: http://www.librecat.org/personId=4800CC20-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2140-7195 - foaf_Person: foaf_givenName: Wim foaf_name: Grunewald, Wim foaf_surname: Grunewald - foaf_Person: foaf_givenName: Mina K foaf_name: Vasileva, Mina K foaf_surname: Vasileva foaf_workInfoHomepage: http://www.librecat.org/personId=3407EB18-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Gergely foaf_name: Molnar, Gergely foaf_surname: Molnar foaf_workInfoHomepage: http://www.librecat.org/personId=34F1AF46-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Ricardo foaf_name: Tejos, Ricardo foaf_surname: Tejos - foaf_Person: foaf_givenName: Markus foaf_name: Schmid, Markus foaf_surname: Schmid - foaf_Person: foaf_givenName: Michael foaf_name: Sauer, Michael foaf_surname: Sauer - foaf_Person: foaf_givenName: Jirí foaf_name: Friml, Jirí foaf_surname: Friml foaf_workInfoHomepage: http://www.librecat.org/personId=4159519E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8302-7596 bibo_doi: 10.1371/journal.pgen.1007177 bibo_issue: '1' bibo_volume: 14 dct_date: 2018^xs_gYear dct_identifier: - UT:000423718600034 dct_language: eng dct_publisher: Public Library of Science@ dct_title: WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity@ ...