---
_id: '499'
abstract:
- lang: eng
  text: Exposure of an isogenic bacterial population to a cidal antibiotic typically
    fails to eliminate a small fraction of refractory cells. Historically, fractional
    killing has been attributed to infrequently dividing or nondividing "persisters."
    Using microfluidic cultures and time-lapse microscopy, we found that Mycobacterium
    smegmatis persists by dividing in the presence of the drug isoniazid (INH). Although
    persistence in these studies was characterized by stable numbers of cells, this
    apparent stability was actually a dynamic state of balanced division and death.
    Single cells expressed catalase-peroxidase (KatG), which activates INH, in stochastic
    pulses that were negatively correlated with cell survival. These behaviors may
    reflect epigenetic effects, because KatG pulsing and death were correlated between
    sibling cells. Selection of lineages characterized by infrequent KatG pulsing
    could allow nonresponsive adaptation during prolonged drug exposure.
article_processing_charge: No
author:
- first_name: Yurichi
  full_name: Wakamoto, Yurichi
  last_name: Wakamoto
- first_name: Neraaj
  full_name: Dhar, Neraaj
  last_name: Dhar
- first_name: Remy P
  full_name: Chait, Remy P
  id: 3464AE84-F248-11E8-B48F-1D18A9856A87
  last_name: Chait
  orcid: 0000-0003-0876-3187
- first_name: Katrin
  full_name: Schneider, Katrin
  last_name: Schneider
- first_name: François
  full_name: Signorino Gelo, François
  last_name: Signorino Gelo
- first_name: Stanislas
  full_name: Leibler, Stanislas
  last_name: Leibler
- first_name: John
  full_name: Mckinney, John
  last_name: Mckinney
citation:
  ama: Wakamoto Y, Dhar N, Chait RP, et al. Dynamic persistence of antibiotic-stressed
    mycobacteria. <i>Science</i>. 2013;339(6115):91-95. doi:<a href="https://doi.org/10.1126/science.1229858">10.1126/science.1229858</a>
  apa: Wakamoto, Y., Dhar, N., Chait, R. P., Schneider, K., Signorino Gelo, F., Leibler,
    S., &#38; Mckinney, J. (2013). Dynamic persistence of antibiotic-stressed mycobacteria.
    <i>Science</i>. American Association for the Advancement of Science. <a href="https://doi.org/10.1126/science.1229858">https://doi.org/10.1126/science.1229858</a>
  chicago: Wakamoto, Yurichi, Neraaj Dhar, Remy P Chait, Katrin Schneider, François
    Signorino Gelo, Stanislas Leibler, and John Mckinney. “Dynamic Persistence of
    Antibiotic-Stressed Mycobacteria.” <i>Science</i>. American Association for the
    Advancement of Science, 2013. <a href="https://doi.org/10.1126/science.1229858">https://doi.org/10.1126/science.1229858</a>.
  ieee: Y. Wakamoto <i>et al.</i>, “Dynamic persistence of antibiotic-stressed mycobacteria,”
    <i>Science</i>, vol. 339, no. 6115. American Association for the Advancement of
    Science, pp. 91–95, 2013.
  ista: Wakamoto Y, Dhar N, Chait RP, Schneider K, Signorino Gelo F, Leibler S, Mckinney
    J. 2013. Dynamic persistence of antibiotic-stressed mycobacteria. Science. 339(6115),
    91–95.
  mla: Wakamoto, Yurichi, et al. “Dynamic Persistence of Antibiotic-Stressed Mycobacteria.”
    <i>Science</i>, vol. 339, no. 6115, American Association for the Advancement of
    Science, 2013, pp. 91–95, doi:<a href="https://doi.org/10.1126/science.1229858">10.1126/science.1229858</a>.
  short: Y. Wakamoto, N. Dhar, R.P. Chait, K. Schneider, F. Signorino Gelo, S. Leibler,
    J. Mckinney, Science 339 (2013) 91–95.
date_created: 2018-12-11T11:46:48Z
date_published: 2013-01-04T00:00:00Z
date_updated: 2025-09-30T07:29:30Z
day: '04'
department:
- _id: CaGu
- _id: GaTk
doi: 10.1126/science.1229858
external_id:
  isi:
  - '000312985800059'
intvolume: '       339'
isi: 1
issue: '6115'
language:
- iso: eng
month: '01'
oa_version: None
page: 91 - 95
publication: Science
publication_status: published
publisher: American Association for the Advancement of Science
publist_id: '7321'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Dynamic persistence of antibiotic-stressed mycobacteria
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 339
year: '2013'
...