---
OA_place: publisher
_id: '50'
abstract:
- lang: eng
  text: The Wnt/planar cell polarity (Wnt/PCP) pathway determines planar polarity
    of epithelial cells in both vertebrates and invertebrates. The role that Wnt/PCP
    signaling plays in mesenchymal contexts, however, is only poorly understood. While
    previous studies have demonstrated the capacity of Wnt/PCP signaling to polarize
    and guide directed migration of mesenchymal cells, it remains unclear whether
    endogenous Wnt/PCP signaling performs these functions instructively, as it does
    in epithelial cells. Here we developed a light-switchable version of the Wnt/PCP
    receptor Frizzled 7 (Fz7) to unambiguously distinguish between an instructive
    and a permissive role of Wnt/PCP signaling for the directional collective migration
    of mesendoderm progenitor cells during zebrafish gastrulation. We show that prechordal
    plate (ppl) cell migration is defective in maternal-zygotic fz7a and fz7b (MZ
    fz7a,b) double mutant embryos, and that Fz7 functions cell-autonomously in this
    process by promoting ppl cell protrusion formation and directed migration. We
    further show that local activation of Fz7 can direct ppl cell migration both in
    vitro and in vivo. Surprisingly, however, uniform Fz7 activation is sufficient
    to fully rescue the ppl cell migration defect in MZ fz7a,b mutant embryos, indicating
    that Wnt/PCP signaling functions permissively rather than instructively in directed
    mesendoderm cell migration during zebrafish gastrulation.
alternative_title:
- ISTA Thesis
article_processing_charge: No
author:
- first_name: Daniel
  full_name: Capek, Daniel
  id: 31C42484-F248-11E8-B48F-1D18A9856A87
  last_name: Capek
  orcid: 0000-0001-5199-9940
citation:
  ama: Capek D. Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling
    in directed mesenchymal cell migration. 2018. doi:<a href="https://doi.org/10.15479/AT:ISTA:TH_1031">10.15479/AT:ISTA:TH_1031</a>
  apa: Capek, D. (2018). <i>Optogenetic Frizzled 7 reveals a permissive function of
    Wnt/PCP signaling in directed mesenchymal cell migration</i>. Institute of Science
    and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:TH_1031">https://doi.org/10.15479/AT:ISTA:TH_1031</a>
  chicago: Capek, Daniel. “Optogenetic Frizzled 7 Reveals a Permissive Function of
    Wnt/PCP Signaling in Directed Mesenchymal Cell Migration.” Institute of Science
    and Technology Austria, 2018. <a href="https://doi.org/10.15479/AT:ISTA:TH_1031">https://doi.org/10.15479/AT:ISTA:TH_1031</a>.
  ieee: D. Capek, “Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP
    signaling in directed mesenchymal cell migration,” Institute of Science and Technology
    Austria, 2018.
  ista: Capek D. 2018. Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP
    signaling in directed mesenchymal cell migration. Institute of Science and Technology
    Austria.
  mla: Capek, Daniel. <i>Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP
    Signaling in Directed Mesenchymal Cell Migration</i>. Institute of Science and
    Technology Austria, 2018, doi:<a href="https://doi.org/10.15479/AT:ISTA:TH_1031">10.15479/AT:ISTA:TH_1031</a>.
  short: D. Capek, Optogenetic Frizzled 7 Reveals a Permissive Function of Wnt/PCP
    Signaling in Directed Mesenchymal Cell Migration, Institute of Science and Technology
    Austria, 2018.
corr_author: '1'
date_created: 2018-12-11T11:44:21Z
date_published: 2018-06-22T00:00:00Z
date_updated: 2026-04-16T10:07:33Z
day: '22'
ddc:
- '570'
- '591'
- '596'
degree_awarded: PhD
department:
- _id: CaHe
doi: 10.15479/AT:ISTA:TH_1031
file:
- access_level: open_access
  checksum: d3eca3dcacb67bffdde6e6609c31cdd0
  content_type: application/pdf
  creator: dernst
  date_created: 2019-04-08T13:42:26Z
  date_updated: 2021-02-11T11:17:17Z
  embargo: 2019-06-25
  file_id: '6238'
  file_name: 2018_Thesis_Capek.pdf
  file_size: 31576521
  relation: main_file
- access_level: closed
  checksum: 876deb14067e638aba65d209668bd821
  content_type: application/vnd.openxmlformats-officedocument.wordprocessingml.document
  creator: dernst
  date_created: 2019-04-08T13:42:27Z
  date_updated: 2021-02-11T23:30:21Z
  embargo_to: open_access
  file_id: '6239'
  file_name: 2018_Thesis_Capek_source.docx
  file_size: 38992956
  relation: source_file
file_date_updated: 2021-02-11T23:30:21Z
has_accepted_license: '1'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
page: '95'
publication_identifier:
  issn:
  - 2663-337X
publication_status: published
publisher: Institute of Science and Technology Austria
publist_id: '8004'
pubrep_id: '1031'
related_material:
  record:
  - id: '661'
    relation: part_of_dissertation
    status: public
  - id: '1100'
    relation: part_of_dissertation
    status: public
  - id: '676'
    relation: part_of_dissertation
    status: public
status: public
supervisor:
- first_name: Carl-Philipp J
  full_name: Heisenberg, Carl-Philipp J
  id: 39427864-F248-11E8-B48F-1D18A9856A87
  last_name: Heisenberg
  orcid: 0000-0002-0912-4566
title: Optogenetic Frizzled 7 reveals a permissive function of Wnt/PCP signaling in
  directed mesenchymal cell migration
type: dissertation
user_id: ba8df636-2132-11f1-aed0-ed93e2281fdd
year: '2018'
...