@misc{5565,
  abstract     = {One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. 
The Video is licensed under a CC BY NC ND license. },
  author       = {Von Wangenheim, Daniel and Hauschild, Robert and Friml, Jirí},
  publisher    = {Institute of Science and Technology Austria},
  title        = {{Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel}},
  doi          = {10.15479/AT:ISTA:66},
  year         = {2017},
}