{"file_date_updated":"2020-07-14T12:47:03Z","publisher":"Institute of Science and Technology Austria","month":"04","date_published":"2017-04-10T00:00:00Z","date_updated":"2024-02-21T13:49:13Z","title":"Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel","status":"public","has_accepted_license":"1","file":[{"relation":"main_file","file_size":101497758,"checksum":"b7552fc23540a85dc5a22fd4484eae71","creator":"system","content_type":"video/mp4","date_created":"2018-12-12T13:02:33Z","file_name":"IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4","access_level":"open_access","date_updated":"2020-07-14T12:47:03Z","file_id":"5599"}],"oa":1,"datarep_id":"66","department":[{"_id":"JiFr"},{"_id":"Bio"}],"_id":"5565","abstract":[{"text":"One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. \r\nThe Video is licensed under a CC BY NC ND license. ","lang":"eng"}],"ec_funded":1,"acknowledgement":"fund: FP7-ERC 0101109","day":"10","ddc":["580"],"date_created":"2018-12-12T12:31:34Z","project":[{"call_identifier":"FP7","grant_number":"291734","_id":"25681D80-B435-11E9-9278-68D0E5697425","name":"International IST Postdoc Fellowship Programme"}],"doi":"10.15479/AT:ISTA:66","oa_version":"Published Version","article_processing_charge":"No","publist_id":"6302","year":"2017","related_material":{"record":[{"status":"public","id":"1078","relation":"research_paper"}]},"citation":{"ama":"von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. 2017. doi:<a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>","chicago":"Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute of Science and Technology Austria, 2017. <a href=\"https://doi.org/10.15479/AT:ISTA:66\">https://doi.org/10.15479/AT:ISTA:66</a>.","ista":"von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel, Institute of Science and Technology Austria, <a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>.","mla":"von Wangenheim, Daniel, et al. <i>Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel</i>. Institute of Science and Technology Austria, 2017, doi:<a href=\"https://doi.org/10.15479/AT:ISTA:66\">10.15479/AT:ISTA:66</a>.","apa":"von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. Institute of Science and Technology Austria. <a href=\"https://doi.org/10.15479/AT:ISTA:66\">https://doi.org/10.15479/AT:ISTA:66</a>","short":"D. von Wangenheim, R. Hauschild, J. Friml, (2017).","ieee":"D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel.” Institute of Science and Technology Austria, 2017."},"author":[{"orcid":"0000-0002-6862-1247","id":"49E91952-F248-11E8-B48F-1D18A9856A87","first_name":"Daniel","last_name":"Von Wangenheim","full_name":"Von Wangenheim, Daniel"},{"orcid":"0000-0001-9843-3522","last_name":"Hauschild","full_name":"Hauschild, Robert","id":"4E01D6B4-F248-11E8-B48F-1D18A9856A87","first_name":"Robert"},{"first_name":"Jirí","id":"4159519E-F248-11E8-B48F-1D18A9856A87","last_name":"Friml","full_name":"Friml, Jirí","orcid":"0000-0002-8302-7596"}],"type":"research_data","user_id":"2DF688A6-F248-11E8-B48F-1D18A9856A87"}