Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel

database Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel Daniel Von Wangenheim author 49E91952-F248-11E8-B48F-1D18A9856A870000-0002-6862-1247 Robert Hauschild author 4E01D6B4-F248-11E8-B48F-1D18A9856A870000-0001-9843-3522 Jirí Friml author 4159519E-F248-11E8-B48F-1D18A9856A870000-0002-8302-7596 JiFr department Bio department International IST Postdoc Fellowship Programme project One of the key questions in understanding plant development is how single cells behave in a larger context of the tissue. Therefore, it requires the observation of the whole organ with a high spatial- as well as temporal resolution over prolonged periods of time, which may cause photo-toxic effects. This protocol shows a plant sample preparation method for light-sheet microscopy, which is characterized by mounting the plant vertically on the surface of a gel. The plant is mounted in such a way that the roots are submerged in a liquid medium while the leaves remain in the air. In order to ensure photosynthetic activity of the plant, a custom-made lighting system illuminates the leaves. To keep the roots in darkness the water surface is covered with sheets of black plastic foil. This method allows long-term imaging of plant organ development in standardized conditions. The Video is licensed under a CC BY NC ND license. https://research-explorer.ista.ac.at/download/5565/5599/IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4 video/mp4no Institute of Science and Technology Austria2017 10.15479/AT:ISTA:66 https://research-explorer.ista.ac.at/record/1078 D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel.” Institute of Science and Technology Austria, 2017. von Wangenheim, D., Hauschild, R., & Friml, J. (2017). Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. Institute of Science and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:66">https://doi.org/10.15479/AT:ISTA:66</a> von Wangenheim, Daniel, et al. <i>Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel</i>. Institute of Science and Technology Austria, 2017, doi:<a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a>. D. von Wangenheim, R. Hauschild, J. Friml, (2017). von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel, Institute of Science and Technology Austria, <a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a>. Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute of Science and Technology Austria, 2017. <a href="https://doi.org/10.15479/AT:ISTA:66">https://doi.org/10.15479/AT:ISTA:66</a>. von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy of plant roots growing on the surface of a gel. 2017. doi:<a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a> 55652018-12-12T12:31:34Z2026-08-12T14:11:07Z