---
_id: '5565'
abstract:
- lang: eng
  text: "One of the key questions in understanding plant development is how single
    cells behave in a larger context of the tissue. Therefore, it requires the observation
    of the whole organ with a high spatial- as well as temporal resolution over prolonged
    periods of time, which may cause photo-toxic effects. This protocol shows a plant
    sample preparation method for light-sheet microscopy, which is characterized by
    mounting the plant vertically on the surface of a gel. The plant is mounted in
    such a way that the roots are submerged in a liquid medium while the leaves remain
    in the air. In order to ensure photosynthetic activity of the plant, a custom-made
    lighting system illuminates the leaves. To keep the roots in darkness the water
    surface is covered with sheets of black plastic foil. This method allows long-term
    imaging of plant organ development in standardized conditions. \r\nThe Video is
    licensed under a CC BY NC ND license. "
acknowledgement: 'fund: FP7-ERC 0101109'
article_processing_charge: No
author:
- first_name: Daniel
  full_name: Von Wangenheim, Daniel
  id: 49E91952-F248-11E8-B48F-1D18A9856A87
  last_name: Von Wangenheim
  orcid: 0000-0002-6862-1247
- first_name: Robert
  full_name: Hauschild, Robert
  id: 4E01D6B4-F248-11E8-B48F-1D18A9856A87
  last_name: Hauschild
  orcid: 0000-0001-9843-3522
- first_name: Jirí
  full_name: Friml, Jirí
  id: 4159519E-F248-11E8-B48F-1D18A9856A87
  last_name: Friml
  orcid: 0000-0002-8302-7596
citation:
  ama: von Wangenheim D, Hauschild R, Friml J. Light Sheet Fluorescence microscopy
    of plant roots growing on the surface of a gel. 2017. doi:<a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a>
  apa: von Wangenheim, D., Hauschild, R., &#38; Friml, J. (2017). Light Sheet Fluorescence
    microscopy of plant roots growing on the surface of a gel. Institute of Science
    and Technology Austria. <a href="https://doi.org/10.15479/AT:ISTA:66">https://doi.org/10.15479/AT:ISTA:66</a>
  chicago: Wangenheim, Daniel von, Robert Hauschild, and Jiří Friml. “Light Sheet
    Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel.” Institute
    of Science and Technology Austria, 2017. <a href="https://doi.org/10.15479/AT:ISTA:66">https://doi.org/10.15479/AT:ISTA:66</a>.
  ieee: D. von Wangenheim, R. Hauschild, and J. Friml, “Light Sheet Fluorescence microscopy
    of plant roots growing on the surface of a gel.” Institute of Science and Technology
    Austria, 2017.
  ista: von Wangenheim D, Hauschild R, Friml J. 2017. Light Sheet Fluorescence microscopy
    of plant roots growing on the surface of a gel, Institute of Science and Technology
    Austria, <a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a>.
  mla: von Wangenheim, Daniel, et al. <i>Light Sheet Fluorescence Microscopy of Plant
    Roots Growing on the Surface of a Gel</i>. Institute of Science and Technology
    Austria, 2017, doi:<a href="https://doi.org/10.15479/AT:ISTA:66">10.15479/AT:ISTA:66</a>.
  short: D. von Wangenheim, R. Hauschild, J. Friml, (2017).
datarep_id: '66'
date_created: 2018-12-12T12:31:34Z
date_published: 2017-04-10T00:00:00Z
date_updated: 2026-08-12T14:11:07Z
day: '10'
ddc:
- '580'
department:
- _id: JiFr
- _id: Bio
doi: 10.15479/AT:ISTA:66
ec_funded: 1
file:
- access_level: open_access
  checksum: b7552fc23540a85dc5a22fd4484eae71
  content_type: video/mp4
  creator: system
  date_created: 2018-12-12T13:02:33Z
  date_updated: 2020-07-14T12:47:03Z
  file_id: '5599'
  file_name: IST-2017-66-v1+1_WangenheimHighResolution55044-NEW_1.mp4
  file_size: 101497758
  relation: main_file
file_date_updated: 2020-07-14T12:47:03Z
fulldoi: https://doi.org/10.15479/AT:ISTA:66
has_accepted_license: '1'
month: '04'
oa: 1
oa_version: Published Version
project:
- _id: 25681D80-B435-11E9-9278-68D0E5697425
  call_identifier: FP7
  grant_number: '291734'
  name: International IST Postdoc Fellowship Programme
publisher: Institute of Science and Technology Austria
publist_id: '6302'
related_material:
  record:
  - id: '1078'
    relation: research_paper
    status: public
status: public
title: Light Sheet Fluorescence microscopy of plant roots growing on the surface of
  a gel
type: research_data
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
year: '2017'
...