--- res: bibo_abstract: - Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type synaptic terminals are formed. This chapter also presents detailed protocols for utilizing the main technical advantages provided by such a preparation, namely, labeling and imaging of synaptic organelles and electrophysiological recordings directly from presynaptic terminals.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Dimitar foaf_name: Dimitrov, Dimitar foaf_surname: Dimitrov - foaf_Person: foaf_givenName: Laurent foaf_name: Guillaud, Laurent foaf_surname: Guillaud - foaf_Person: foaf_givenName: Kohgaku foaf_name: Eguchi, Kohgaku foaf_surname: Eguchi foaf_workInfoHomepage: http://www.librecat.org/personId=2B7846DC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-6170-2546 - foaf_Person: foaf_givenName: Tomoyuki foaf_name: Takahashi, Tomoyuki foaf_surname: Takahashi bibo_doi: 10.1007/978-1-4939-7571-6_15 bibo_volume: 1727 dct_date: 2018^xs_gYear dct_language: eng dct_publisher: Springer@ dct_title: Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses@ fabio_hasPubmedId: '29222783' ...