book chapter published yes Dimitar Dimitrov author Laurent Guillaud author Kohgaku Eguchi author 2B7846DC-F248-11E8-B48F-1D18A9856A870000-0002-6170-2546 Tomoyuki Takahashi author Stephen D.Skaper editor RySh department Primary neuronal cell culture preparations are widely used to investigate synaptic functions. This chapter describes a detailed protocol for the preparation of a neuronal cell culture in which giant calyx-type synaptic terminals are formed. This chapter also presents detailed protocols for utilizing the main technical advantages provided by such a preparation, namely, labeling and imaging of synaptic organelles and electrophysiological recordings directly from presynaptic terminals. https://research-explorer.ista.ac.at/download/562/7046/2018_NeurotrophicFactors_Dimitrov.pdf application/pdfno Springer2018 eng 2922278310.1007/978-1-4939-7571-6_15 1727201 - 215 Dimitrov, Dimitar, et al. “Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses.” <i>Neurotrophic Factors</i>, edited by Stephen D. Skaper, vol. 1727, Springer, 2018, pp. 201–15, doi:<a href="https://doi.org/10.1007/978-1-4939-7571-6_15">10.1007/978-1-4939-7571-6_15</a>. D. Dimitrov, L. Guillaud, K. Eguchi, T. Takahashi, in:, S.D. Skaper (Ed.), Neurotrophic Factors, Springer, 2018, pp. 201–215. Dimitrov, D., Guillaud, L., Eguchi, K., &#38; Takahashi, T. (2018). Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses. In S. D. Skaper (Ed.), <i>Neurotrophic Factors</i> (Vol. 1727, pp. 201–215). Springer. <a href="https://doi.org/10.1007/978-1-4939-7571-6_15">https://doi.org/10.1007/978-1-4939-7571-6_15</a> Dimitrov, Dimitar, Laurent Guillaud, Kohgaku Eguchi, and Tomoyuki Takahashi. “Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging and Electrophysiological Analyses.” In <i>Neurotrophic Factors</i>, edited by Stephen D. Skaper, 1727:201–15. Springer, 2018. <a href="https://doi.org/10.1007/978-1-4939-7571-6_15">https://doi.org/10.1007/978-1-4939-7571-6_15</a>. Dimitrov D, Guillaud L, Eguchi K, Takahashi T. Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses. In: Skaper SD, ed. <i>Neurotrophic Factors</i>. Vol 1727. Springer; 2018:201-215. doi:<a href="https://doi.org/10.1007/978-1-4939-7571-6_15">10.1007/978-1-4939-7571-6_15</a> Dimitrov D, Guillaud L, Eguchi K, Takahashi T. 2018.Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses. In: Neurotrophic Factors. Methods in Molecular Biology, vol. 1727, 201–215. D. Dimitrov, L. Guillaud, K. Eguchi, and T. Takahashi, “Culture of mouse giant central nervous system synapses and application for imaging and electrophysiological analyses,” in <i>Neurotrophic Factors</i>, vol. 1727, S. D. Skaper, Ed. Springer, 2018, pp. 201–215. 5622018-12-11T11:47:11Z2021-01-12T08:03:05Z