---
_id: '562'
abstract:
- lang: eng
  text: Primary neuronal cell culture preparations are widely used to investigate
    synaptic functions. This chapter describes a detailed protocol for the preparation
    of a neuronal cell culture in which giant calyx-type synaptic terminals are formed.
    This chapter also presents detailed protocols for utilizing the main technical
    advantages provided by such a preparation, namely, labeling and imaging of synaptic
    organelles and electrophysiological recordings directly from presynaptic terminals.
alternative_title:
- Methods in Molecular Biology
article_processing_charge: No
author:
- first_name: Dimitar
  full_name: Dimitrov, Dimitar
  last_name: Dimitrov
- first_name: Laurent
  full_name: Guillaud, Laurent
  last_name: Guillaud
- first_name: Kohgaku
  full_name: Eguchi, Kohgaku
  id: 2B7846DC-F248-11E8-B48F-1D18A9856A87
  last_name: Eguchi
  orcid: 0000-0002-6170-2546
- first_name: Tomoyuki
  full_name: Takahashi, Tomoyuki
  last_name: Takahashi
citation:
  ama: 'Dimitrov D, Guillaud L, Eguchi K, Takahashi T. Culture of mouse giant central
    nervous system synapses and application for imaging and electrophysiological analyses.
    In: Skaper SD, ed. <i>Neurotrophic Factors</i>. Vol 1727. Springer; 2018:201-215.
    doi:<a href="https://doi.org/10.1007/978-1-4939-7571-6_15">10.1007/978-1-4939-7571-6_15</a>'
  apa: Dimitrov, D., Guillaud, L., Eguchi, K., &#38; Takahashi, T. (2018). Culture
    of mouse giant central nervous system synapses and application for imaging and
    electrophysiological analyses. In S. D. Skaper (Ed.), <i>Neurotrophic Factors</i>
    (Vol. 1727, pp. 201–215). Springer. <a href="https://doi.org/10.1007/978-1-4939-7571-6_15">https://doi.org/10.1007/978-1-4939-7571-6_15</a>
  chicago: Dimitrov, Dimitar, Laurent Guillaud, Kohgaku Eguchi, and Tomoyuki Takahashi.
    “Culture of Mouse Giant Central Nervous System Synapses and Application for Imaging
    and Electrophysiological Analyses.” In <i>Neurotrophic Factors</i>, edited by
    Stephen D. Skaper, 1727:201–15. Springer, 2018. <a href="https://doi.org/10.1007/978-1-4939-7571-6_15">https://doi.org/10.1007/978-1-4939-7571-6_15</a>.
  ieee: D. Dimitrov, L. Guillaud, K. Eguchi, and T. Takahashi, “Culture of mouse giant
    central nervous system synapses and application for imaging and electrophysiological
    analyses,” in <i>Neurotrophic Factors</i>, vol. 1727, S. D. Skaper, Ed. Springer,
    2018, pp. 201–215.
  ista: 'Dimitrov D, Guillaud L, Eguchi K, Takahashi T. 2018.Culture of mouse giant
    central nervous system synapses and application for imaging and electrophysiological
    analyses. In: Neurotrophic Factors. Methods in Molecular Biology, vol. 1727, 201–215.'
  mla: Dimitrov, Dimitar, et al. “Culture of Mouse Giant Central Nervous System Synapses
    and Application for Imaging and Electrophysiological Analyses.” <i>Neurotrophic
    Factors</i>, edited by Stephen D. Skaper, vol. 1727, Springer, 2018, pp. 201–15,
    doi:<a href="https://doi.org/10.1007/978-1-4939-7571-6_15">10.1007/978-1-4939-7571-6_15</a>.
  short: D. Dimitrov, L. Guillaud, K. Eguchi, T. Takahashi, in:, S.D. Skaper (Ed.),
    Neurotrophic Factors, Springer, 2018, pp. 201–215.
date_created: 2018-12-11T11:47:11Z
date_published: 2018-01-01T00:00:00Z
date_updated: 2021-01-12T08:03:05Z
day: '01'
ddc:
- '570'
department:
- _id: RySh
doi: 10.1007/978-1-4939-7571-6_15
editor:
- first_name: Stephen D.
  full_name: Skaper, Stephen D.
  last_name: Skaper
external_id:
  pmid:
  - '29222783'
file:
- access_level: open_access
  checksum: 8aa174ca65a56fbb19e9f88cff3ac3fd
  content_type: application/pdf
  creator: dernst
  date_created: 2019-11-19T07:47:43Z
  date_updated: 2020-07-14T12:47:09Z
  file_id: '7046'
  file_name: 2018_NeurotrophicFactors_Dimitrov.pdf
  file_size: 787407
  relation: main_file
file_date_updated: 2020-07-14T12:47:09Z
has_accepted_license: '1'
intvolume: '      1727'
language:
- iso: eng
month: '01'
oa: 1
oa_version: Submitted Version
page: 201 - 215
pmid: 1
publication: Neurotrophic Factors
publication_status: published
publisher: Springer
publist_id: '7252'
quality_controlled: '1'
scopus_import: 1
status: public
title: Culture of mouse giant central nervous system synapses and application for
  imaging and electrophysiological analyses
type: book_chapter
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 1727
year: '2018'
...