---
_id: '5908'
abstract:
- lang: eng
text: The interorganelle communication mediated by membrane contact sites (MCSs)
is an evolutionary hallmark of eukaryotic cells. MCS connections enable the nonvesicular
exchange of information between organelles and allow them to coordinate responses
to changing cellular environments. In plants, the importance of MCS components
in the responses to environmental stress has been widely established, but the
molecular mechanisms regulating interorganelle connectivity during stress still
remain opaque. In this report, we use the model plant Arabidopsis thaliana to
show that ionic stress increases endoplasmic reticulum (ER)–plasma membrane (PM)
connectivity by promoting the cortical expansion of synaptotagmin 1 (SYT1)-enriched
ER–PM contact sites (S-EPCSs). We define differential roles for the cortical cytoskeleton
in the regulation of S-EPCS dynamics and ER–PM connectivity, and we identify the
accumulation of phosphatidylinositol 4,5-bisphosphate [PI(4,5)P2] at the PM as
a molecular signal associated with the ER–PM connectivity changes. Our study highlights
the functional conservation of EPCS components and PM phosphoinositides as modulators
of ER–PM connectivity in eukaryotes, and uncovers unique aspects of the spatiotemporal
regulation of ER–PM connectivity in plants.
article_processing_charge: No
article_type: original
author:
- first_name: Eunkyoung
full_name: Lee, Eunkyoung
last_name: Lee
- first_name: Steffen
full_name: Vanneste, Steffen
last_name: Vanneste
- first_name: Jessica
full_name: Pérez-Sancho, Jessica
last_name: Pérez-Sancho
- first_name: Francisco
full_name: Benitez-Fuente, Francisco
last_name: Benitez-Fuente
- first_name: Matthew
full_name: Strelau, Matthew
last_name: Strelau
- first_name: Alberto P.
full_name: Macho, Alberto P.
last_name: Macho
- first_name: Miguel A.
full_name: Botella, Miguel A.
last_name: Botella
- first_name: Jiří
full_name: Friml, Jiří
id: 4159519E-F248-11E8-B48F-1D18A9856A87
last_name: Friml
orcid: 0000-0002-8302-7596
- first_name: Abel
full_name: Rosado, Abel
last_name: Rosado
citation:
ama: Lee E, Vanneste S, Pérez-Sancho J, et al. Ionic stress enhances ER–PM connectivity
via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. Proceedings
of the National Academy of Sciences of the United States of America. 2019;116(4):1420-1429.
doi:10.1073/pnas.1818099116
apa: Lee, E., Vanneste, S., Pérez-Sancho, J., Benitez-Fuente, F., Strelau, M., Macho,
A. P., … Rosado, A. (2019). Ionic stress enhances ER–PM connectivity via phosphoinositide-associated
SYT1 contact site expansion in Arabidopsis. Proceedings of the National Academy
of Sciences of the United States of America. National Academy of Sciences.
https://doi.org/10.1073/pnas.1818099116
chicago: Lee, Eunkyoung, Steffen Vanneste, Jessica Pérez-Sancho, Francisco Benitez-Fuente,
Matthew Strelau, Alberto P. Macho, Miguel A. Botella, Jiří Friml, and Abel Rosado.
“Ionic Stress Enhances ER–PM Connectivity via Phosphoinositide-Associated SYT1
Contact Site Expansion in Arabidopsis.” Proceedings of the National Academy
of Sciences of the United States of America. National Academy of Sciences,
2019. https://doi.org/10.1073/pnas.1818099116.
ieee: E. Lee et al., “Ionic stress enhances ER–PM connectivity via phosphoinositide-associated
SYT1 contact site expansion in Arabidopsis,” Proceedings of the National Academy
of Sciences of the United States of America, vol. 116, no. 4. National Academy
of Sciences, pp. 1420–1429, 2019.
ista: Lee E, Vanneste S, Pérez-Sancho J, Benitez-Fuente F, Strelau M, Macho AP,
Botella MA, Friml J, Rosado A. 2019. Ionic stress enhances ER–PM connectivity
via phosphoinositide-associated SYT1 contact site expansion in Arabidopsis. Proceedings
of the National Academy of Sciences of the United States of America. 116(4), 1420–1429.
mla: Lee, Eunkyoung, et al. “Ionic Stress Enhances ER–PM Connectivity via Phosphoinositide-Associated
SYT1 Contact Site Expansion in Arabidopsis.” Proceedings of the National Academy
of Sciences of the United States of America, vol. 116, no. 4, National Academy
of Sciences, 2019, pp. 1420–29, doi:10.1073/pnas.1818099116.
short: E. Lee, S. Vanneste, J. Pérez-Sancho, F. Benitez-Fuente, M. Strelau, A.P.
Macho, M.A. Botella, J. Friml, A. Rosado, Proceedings of the National Academy
of Sciences of the United States of America 116 (2019) 1420–1429.
date_created: 2019-02-03T22:59:14Z
date_published: 2019-01-22T00:00:00Z
date_updated: 2023-08-24T14:31:09Z
day: '22'
department:
- _id: JiFr
doi: 10.1073/pnas.1818099116
external_id:
isi:
- '000456336100050'
pmid:
- '30610176'
intvolume: ' 116'
isi: 1
issue: '4'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://doi.org/10.1073/pnas.1818099116
month: '01'
oa: 1
oa_version: Published Version
page: 1420-1429
pmid: 1
publication: Proceedings of the National Academy of Sciences of the United States
of America
publication_status: published
publisher: National Academy of Sciences
quality_controlled: '1'
scopus_import: '1'
status: public
title: Ionic stress enhances ER–PM connectivity via phosphoinositide-associated SYT1
contact site expansion in Arabidopsis
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 116
year: '2019'
...