---
_id: '596'
abstract:
- lang: eng
text: The human Mediator complex controls RNA polymerase II (pol II) function in
ways that remain incompletely understood. Activator-Mediator binding alters Mediator
structure, and these activator-induced structural shifts appear to play key roles
in regulating transcription. A recent cryo-electron microscopy (EM) analysis revealed
that pol II adopted a stable orientation within a Mediator-pol II-TFIIF assembly
in which Mediator was bound to the activation domain of viral protein 16 (VP16).
Whereas TFIIF was shown to be important for orienting pol II within this assembly,
the potential role of the activator was not assessed. To determine how activator
binding might affect pol II orientation, we isolated human Mediator-pol II-TFIIF
complexes in which Mediator was not bound to an activator. Cryo-EM analysis of
this assembly, coupled with pol II crystal structure docking, revealed that pol
II binds Mediator at the same general location; however, in contrast to VP16-bound
Mediator, pol II does not appear to stably orient in the absence of an activator.
Variability in pol II orientation might be important mechanistically, perhaps
to enable sense and antisense transcription at human promoters. Because Mediator
interacts extensively with pol II, these results suggest that Mediator structural
shifts induced by activator binding help stably orient pol II prior to transcription
initiation.
article_processing_charge: No
author:
- first_name: Carrie A
full_name: Bernecky, Carrie A
id: 2CB9DFE2-F248-11E8-B48F-1D18A9856A87
last_name: Bernecky
orcid: 0000-0003-0893-7036
- first_name: Dylan
full_name: Taatjes, Dylan
last_name: Taatjes
citation:
ama: Bernecky C, Taatjes D. Activator-mediator binding stabilizes RNA polymerase
II orientation within the human mediator-RNA polymerase II-TFIIF assembly. Journal
of Molecular Biology. 2012;417(5):387-394. doi:10.1016/j.jmb.2012.02.014
apa: Bernecky, C., & Taatjes, D. (2012). Activator-mediator binding stabilizes
RNA polymerase II orientation within the human mediator-RNA polymerase II-TFIIF
assembly. Journal of Molecular Biology. Elsevier. https://doi.org/10.1016/j.jmb.2012.02.014
chicago: Bernecky, Carrie, and Dylan Taatjes. “Activator-Mediator Binding Stabilizes
RNA Polymerase II Orientation within the Human Mediator-RNA Polymerase II-TFIIF
Assembly.” Journal of Molecular Biology. Elsevier, 2012. https://doi.org/10.1016/j.jmb.2012.02.014.
ieee: C. Bernecky and D. Taatjes, “Activator-mediator binding stabilizes RNA polymerase
II orientation within the human mediator-RNA polymerase II-TFIIF assembly,” Journal
of Molecular Biology, vol. 417, no. 5. Elsevier, pp. 387–394, 2012.
ista: Bernecky C, Taatjes D. 2012. Activator-mediator binding stabilizes RNA polymerase
II orientation within the human mediator-RNA polymerase II-TFIIF assembly. Journal
of Molecular Biology. 417(5), 387–394.
mla: Bernecky, Carrie, and Dylan Taatjes. “Activator-Mediator Binding Stabilizes
RNA Polymerase II Orientation within the Human Mediator-RNA Polymerase II-TFIIF
Assembly.” Journal of Molecular Biology, vol. 417, no. 5, Elsevier, 2012,
pp. 387–94, doi:10.1016/j.jmb.2012.02.014.
short: C. Bernecky, D. Taatjes, Journal of Molecular Biology 417 (2012) 387–394.
date_created: 2018-12-11T11:47:24Z
date_published: 2012-04-13T00:00:00Z
date_updated: 2021-01-12T08:05:21Z
day: '13'
doi: 10.1016/j.jmb.2012.02.014
extern: '1'
intvolume: ' 417'
issue: '5'
language:
- iso: eng
main_file_link:
- open_access: '1'
url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4582759/
month: '04'
oa: 1
oa_version: None
page: 387 - 394
publication: Journal of Molecular Biology
publication_status: published
publisher: Elsevier
publist_id: '7208'
status: public
title: Activator-mediator binding stabilizes RNA polymerase II orientation within
the human mediator-RNA polymerase II-TFIIF assembly
type: journal_article
user_id: 2DF688A6-F248-11E8-B48F-1D18A9856A87
volume: 417
year: '2012'
...