---
res:
  bibo_abstract:
  - Sudden stress often triggers diverse, temporally structured gene expression responses
    in microbes, but it is largely unknown how variable in time such responses are
    and if genes respond in the same temporal order in every single cell. Here, we
    quantified timing variability of individual promoters responding to sublethal
    antibiotic stress using fluorescent reporters, microfluidics, and time‐lapse microscopy.
    We identified lower and upper bounds that put definite constraints on timing variability,
    which varies strongly among promoters and conditions. Timing variability can be
    interpreted using results from statistical kinetics, which enable us to estimate
    the number of rate‐limiting molecular steps underlying different responses. We
    found that just a few critical steps control some responses while others rely
    on dozens of steps. To probe connections between different stress responses, we
    then tracked the temporal order and response time correlations of promoter pairs
    in individual cells. Our results support that, when bacteria are exposed to the
    antibiotic nitrofurantoin, the ensuing oxidative stress and SOS responses are
    part of the same causal chain of molecular events. In contrast, under trimethoprim,
    the acid stress response and the SOS response are part of different chains of
    events running in parallel. Our approach reveals fundamental constraints on gene
    expression timing and provides new insights into the molecular events that underlie
    the timing of stress responses.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Karin
      foaf_name: Mitosch, Karin
      foaf_surname: Mitosch
      foaf_workInfoHomepage: http://www.librecat.org/personId=39B66846-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Georg
      foaf_name: Rieckh, Georg
      foaf_surname: Rieckh
      foaf_workInfoHomepage: http://www.librecat.org/personId=34DA8BD6-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Mark Tobias
      foaf_name: Bollenbach, Mark Tobias
      foaf_surname: Bollenbach
      foaf_workInfoHomepage: http://www.librecat.org/personId=3E6DB97A-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-4398-476X
  bibo_doi: 10.15252/msb.20188470
  bibo_issue: '2'
  bibo_volume: 15
  dct_date: 2019^xs_gYear
  dct_identifier:
  - UT:000459628300003
  dct_language: eng
  dct_publisher: Embo Press@
  dct_title: Temporal order and precision of complex stress responses in individual
    bacteria@
  fabio_hasPubmedId: '30765425'
...