---
res:
  bibo_abstract:
  - 'Expansion microscopy is a relatively new approach to super-resolution imaging
    that uses expandable hydrogels to isotropically increase the physical distance
    between fluorophores in biological samples such as cell cultures or tissue slices.
    The classic gel recipe results in an expansion factor of ~4×, with a resolution
    of 60–80 nm. We have recently developed X10 microscopy, which uses a gel that
    achieves an expansion factor of ~10×, with a resolution of ~25 nm. Here, we provide
    a step-by-step protocol for X10 expansion microscopy. A typical experiment consists
    of seven sequential stages: (i) immunostaining, (ii) anchoring, (iii) polymerization,
    (iv) homogenization, (v) expansion, (vi) imaging, and (vii) validation. The protocol
    presented here includes recommendations for optimization, pitfalls and their solutions,
    and detailed guidelines that should increase reproducibility. Although our protocol
    focuses on X10 expansion microscopy, we detail which of these suggestions are
    also applicable to classic fourfold expansion microscopy. We exemplify our protocol
    using primary hippocampal neurons from rats, but our approach can be used with
    other primary cells or cultured cell lines of interest. This protocol will enable
    any researcher with basic experience in immunostainings and access to an epifluorescence
    microscope to perform super-resolution microscopy with X10. The procedure takes
    3 d and requires ~5 h of actively handling the sample for labeling and expansion,
    and another ~3 h for imaging and analysis.@eng'
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Sven M
      foaf_name: Truckenbrodt, Sven M
      foaf_surname: Truckenbrodt
      foaf_workInfoHomepage: http://www.librecat.org/personId=45812BD4-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Christoph M
      foaf_name: Sommer, Christoph M
      foaf_surname: Sommer
      foaf_workInfoHomepage: http://www.librecat.org/personId=4DF26D8C-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-1216-9105
  - foaf_Person:
      foaf_givenName: Silvio O
      foaf_name: Rizzoli, Silvio O
      foaf_surname: Rizzoli
  - foaf_Person:
      foaf_givenName: Johann G
      foaf_name: Danzl, Johann G
      foaf_surname: Danzl
      foaf_workInfoHomepage: http://www.librecat.org/personId=42EFD3B6-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8559-3973
  bibo_doi: 10.1038/s41596-018-0117-3
  bibo_issue: '3'
  bibo_volume: 14
  dct_date: 2019^xs_gYear
  dct_identifier:
  - UT:000459890700008
  dct_language: eng
  dct_publisher: Nature Publishing Group@
  dct_title: A practical guide to optimization in X10 expansion microscopy@
  fabio_hasPubmedId: '30778205'
...