---
_id: '6111'
abstract:
- lang: eng
  text: 'Neurons develop elaborate morphologies that provide a model for understanding
    cellular architecture. By studying C. elegans sensory dendrites, we previously
    identified genes that act to promote the extension of ciliated sensory dendrites
    during embryogenesis. Interestingly, the nonciliated dendrite of the oxygen-sensing
    neuron URX is not affected by these genes, suggesting it develops through a distinct
    mechanism. Here, we use a visual forward genetic screen to identify mutants that
    affect URX dendrite morphogenesis. We find that disruption of the MAP kinase MAPK-15
    or the βH-spectrin SMA-1 causes a phenotype opposite to what we had seen before:
    dendrites extend normally during embryogenesis but begin to overgrow as the animals
    reach adulthood, ultimately extending up to 150% of their normal length. SMA-1
    is broadly expressed and acts non-cell-autonomously, while MAPK-15 is expressed
    in many sensory neurons including URX and acts cell-autonomously. MAPK-15 acts
    at the time of overgrowth, localizes at the dendrite ending, and requires its
    kinase activity, suggesting it acts locally in time and space to constrain dendrite
    growth. Finally, we find that the oxygen-sensing guanylate cyclase GCY-35, which
    normally localizes at the dendrite ending, is localized throughout the overgrown
    region, and that overgrowth can be suppressed by overexpressing GCY-35 or by genetically
    mimicking elevated cGMP signaling. These results suggest that overgrowth may correspond
    to expansion of a sensory compartment at the dendrite ending, reminiscent of the
    remodeling of sensory cilia or dendritic spines. Thus, in contrast to established
    pathways that promote dendrite growth during early development, our results reveal
    a distinct mechanism that constrains dendrite growth throughout the life of the
    animal, possibly by controlling the size of a sensory compartment at the dendrite
    ending.'
article_number: e1007435
author:
- first_name: Ian G.
  full_name: McLachlan, Ian G.
  last_name: McLachlan
- first_name: Isabel
  full_name: Beets, Isabel
  last_name: Beets
- first_name: Mario
  full_name: de Bono, Mario
  id: 4E3FF80E-F248-11E8-B48F-1D18A9856A87
  last_name: de Bono
  orcid: 0000-0001-8347-0443
- first_name: Maxwell G.
  full_name: Heiman, Maxwell G.
  last_name: Heiman
citation:
  ama: McLachlan IG, Beets I, de Bono M, Heiman MG. A neuronal MAP kinase constrains
    growth of a Caenorhabditis elegans sensory dendrite throughout the life of the
    organism. <i>PLOS Genetics</i>. 2018;14(6). doi:<a href="https://doi.org/10.1371/journal.pgen.1007435">10.1371/journal.pgen.1007435</a>
  apa: McLachlan, I. G., Beets, I., de Bono, M., &#38; Heiman, M. G. (2018). A neuronal
    MAP kinase constrains growth of a Caenorhabditis elegans sensory dendrite throughout
    the life of the organism. <i>PLOS Genetics</i>. Public Library of Science. <a
    href="https://doi.org/10.1371/journal.pgen.1007435">https://doi.org/10.1371/journal.pgen.1007435</a>
  chicago: McLachlan, Ian G., Isabel Beets, Mario de Bono, and Maxwell G. Heiman.
    “A Neuronal MAP Kinase Constrains Growth of a Caenorhabditis Elegans Sensory Dendrite
    throughout the Life of the Organism.” <i>PLOS Genetics</i>. Public Library of
    Science, 2018. <a href="https://doi.org/10.1371/journal.pgen.1007435">https://doi.org/10.1371/journal.pgen.1007435</a>.
  ieee: I. G. McLachlan, I. Beets, M. de Bono, and M. G. Heiman, “A neuronal MAP kinase
    constrains growth of a Caenorhabditis elegans sensory dendrite throughout the
    life of the organism,” <i>PLOS Genetics</i>, vol. 14, no. 6. Public Library of
    Science, 2018.
  ista: McLachlan IG, Beets I, de Bono M, Heiman MG. 2018. A neuronal MAP kinase constrains
    growth of a Caenorhabditis elegans sensory dendrite throughout the life of the
    organism. PLOS Genetics. 14(6), e1007435.
  mla: McLachlan, Ian G., et al. “A Neuronal MAP Kinase Constrains Growth of a Caenorhabditis
    Elegans Sensory Dendrite throughout the Life of the Organism.” <i>PLOS Genetics</i>,
    vol. 14, no. 6, e1007435, Public Library of Science, 2018, doi:<a href="https://doi.org/10.1371/journal.pgen.1007435">10.1371/journal.pgen.1007435</a>.
  short: I.G. McLachlan, I. Beets, M. de Bono, M.G. Heiman, PLOS Genetics 14 (2018).
date_created: 2019-03-19T13:09:28Z
date_published: 2018-06-07T00:00:00Z
date_updated: 2021-01-12T08:06:11Z
day: '07'
ddc:
- '570'
doi: 10.1371/journal.pgen.1007435
extern: '1'
external_id:
  pmid:
  - '29879119'
file:
- access_level: open_access
  checksum: 622036b945365dbc575bea2768aa9bc8
  content_type: application/pdf
  creator: kschuh
  date_created: 2019-03-19T13:18:01Z
  date_updated: 2020-07-14T12:47:19Z
  file_id: '6112'
  file_name: 2018_PLOS_McLachlan.pdf
  file_size: 13011506
  relation: main_file
file_date_updated: 2020-07-14T12:47:19Z
has_accepted_license: '1'
intvolume: '        14'
issue: '6'
language:
- iso: eng
month: '06'
oa: 1
oa_version: Published Version
pmid: 1
publication: PLOS Genetics
publication_identifier:
  issn:
  - 1553-7404
publication_status: published
publisher: Public Library of Science
quality_controlled: '1'
status: public
title: A neuronal MAP kinase constrains growth of a Caenorhabditis elegans sensory
  dendrite throughout the life of the organism
tmp:
  image: /images/cc_by.png
  legal_code_url: https://creativecommons.org/licenses/by/4.0/legalcode
  name: Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)
  short: CC BY (4.0)
type: journal_article
user_id: 3E5EF7F0-F248-11E8-B48F-1D18A9856A87
volume: 14
year: '2018'
...