---
res:
  bibo_abstract:
  - Metabotropic GABAB receptors mediate slow inhibitory effects presynaptically and
    postsynaptically through the modulation of different effector signalling pathways.
    Here, we analysed the distribution of GABAB receptors using highly sensitive SDS-digested
    freeze-fracture replica labelling in mouse cerebellar Purkinje cells. Immunoreactivity
    for GABAB1 was observed on presynaptic and, more abundantly, on postsynaptic compartments,
    showing both scattered and clustered distribution patterns. Quantitative analysis
    of immunoparticles revealed a somato-dendritic gradient, with the density of immunoparticles
    increasing 26-fold from somata to dendritic spines. To understand the spatial
    relationship of GABAB receptors with two key effector ion channels, the G protein-gated
    inwardly rectifying K+ (GIRK/Kir3) channel and the voltage-dependent Ca2+ channel,
    biochemical and immunohistochemical approaches were performed. Co-immunoprecipitation
    analysis demonstrated that GABAB receptors co-assembled with GIRK and CaV2.1 channels
    in the cerebellum. Using double-labelling immunoelectron microscopic techniques,
    co-clustering between GABAB1 and GIRK2 was detected in dendritic spines, whereas
    they were mainly segregated in the dendritic shafts. In contrast, co-clustering
    of GABAB1 and CaV2.1 was detected in dendritic shafts but not spines. Presynaptically,
    although no significant co-clustering of GABAB1 and GIRK2 or CaV2.1 channels was
    detected, inter-cluster distance for GABAB1 and GIRK2 was significantly smaller
    in the active zone than in the dendritic shafts, and that for GABAB1 and CaV2.1
    was significantly smaller in the active zone than in the dendritic shafts and
    spines. Thus, GABAB receptors are associated with GIRK and CaV2.1 channels in
    different subcellular compartments. These data provide a better framework for
    understanding the different roles played by GABAB receptors and their effector
    ion channels in the cerebellar network.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Rafael
      foaf_name: Luján, Rafael
      foaf_surname: Luján
  - foaf_Person:
      foaf_givenName: Carolina
      foaf_name: Aguado, Carolina
      foaf_surname: Aguado
  - foaf_Person:
      foaf_givenName: Francisco
      foaf_name: Ciruela, Francisco
      foaf_surname: Ciruela
  - foaf_Person:
      foaf_givenName: Javier
      foaf_name: Cózar, Javier
      foaf_surname: Cózar
  - foaf_Person:
      foaf_givenName: David
      foaf_name: Kleindienst, David
      foaf_surname: Kleindienst
      foaf_workInfoHomepage: http://www.librecat.org/personId=42E121A4-F248-11E8-B48F-1D18A9856A87
  - foaf_Person:
      foaf_givenName: Luis
      foaf_name: De La Ossa, Luis
      foaf_surname: De La Ossa
  - foaf_Person:
      foaf_givenName: Bernhard
      foaf_name: Bettler, Bernhard
      foaf_surname: Bettler
  - foaf_Person:
      foaf_givenName: Kevin
      foaf_name: Wickman, Kevin
      foaf_surname: Wickman
  - foaf_Person:
      foaf_givenName: Masahiko
      foaf_name: Watanabe, Masahiko
      foaf_surname: Watanabe
  - foaf_Person:
      foaf_givenName: Ryuichi
      foaf_name: Shigemoto, Ryuichi
      foaf_surname: Shigemoto
      foaf_workInfoHomepage: http://www.librecat.org/personId=499F3ABC-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0001-8761-9444
  - foaf_Person:
      foaf_givenName: Yugo
      foaf_name: Fukazawa, Yugo
      foaf_surname: Fukazawa
  bibo_doi: 10.1007/s00429-017-1568-y
  bibo_issue: '3'
  bibo_volume: 223
  dct_date: 2018^xs_gYear
  dct_identifier:
  - UT:000428419500030
  dct_language: eng
  dct_publisher: Springer@
  dct_title: Differential association of GABAB receptors with their effector ion channels
    in Purkinje cells@
...