--- res: bibo_abstract: - Aberrant display of the truncated core1 O-glycan T-antigen is a common feature of human cancer cells that correlates with metastasis. Here we show that T-antigen in Drosophila melanogaster macrophages is involved in their developmentally programmed tissue invasion. Higher macrophage T-antigen levels require an atypical major facilitator superfamily (MFS) member that we named Minerva which enables macrophage dissemination and invasion. We characterize for the first time the T and Tn glycoform O-glycoproteome of the Drosophila melanogaster embryo, and determine that Minerva increases the presence of T-antigen on proteins in pathways previously linked to cancer, most strongly on the sulfhydryl oxidase Qsox1 which we show is required for macrophage tissue entry. Minerva’s vertebrate ortholog, MFSD1, rescues the minerva mutant’s migration and T-antigen glycosylation defects. We thus identify a key conserved regulator that orchestrates O-glycosylation on a protein subset to activate a program governing migration steps important for both development and cancer metastasis.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Katarina foaf_name: Valosková, Katarina foaf_surname: Valosková foaf_workInfoHomepage: http://www.librecat.org/personId=46F146FC-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Julia foaf_name: Biebl, Julia foaf_surname: Biebl foaf_workInfoHomepage: http://www.librecat.org/personId=3CCBB46E-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Marko foaf_name: Roblek, Marko foaf_surname: Roblek foaf_workInfoHomepage: http://www.librecat.org/personId=3047D808-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-9588-1389 - foaf_Person: foaf_givenName: Shamsi foaf_name: Emtenani, Shamsi foaf_surname: Emtenani foaf_workInfoHomepage: http://www.librecat.org/personId=49D32318-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6981-6938 - foaf_Person: foaf_givenName: Attila foaf_name: György, Attila foaf_surname: György foaf_workInfoHomepage: http://www.librecat.org/personId=3BCEDBE0-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-1819-198X - foaf_Person: foaf_givenName: Michaela foaf_name: Misova, Michaela foaf_surname: Misova foaf_workInfoHomepage: http://www.librecat.org/personId=495A3C32-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0003-2427-6856 - foaf_Person: foaf_givenName: Aparna foaf_name: Ratheesh, Aparna foaf_surname: Ratheesh foaf_workInfoHomepage: http://www.librecat.org/personId=2F064CFE-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-7190-0776 - foaf_Person: foaf_givenName: Patricia foaf_name: Rodrigues, Patricia foaf_surname: Rodrigues foaf_workInfoHomepage: http://www.librecat.org/personId=2CE4065A-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Katerina foaf_name: Shkarina, Katerina foaf_surname: Shkarina - foaf_Person: foaf_givenName: Ida Signe Bohse foaf_name: Larsen, Ida Signe Bohse foaf_surname: Larsen - foaf_Person: foaf_givenName: Sergey Y foaf_name: Vakhrushev, Sergey Y foaf_surname: Vakhrushev - foaf_Person: foaf_givenName: Henrik foaf_name: Clausen, Henrik foaf_surname: Clausen - foaf_Person: foaf_givenName: Daria E foaf_name: Siekhaus, Daria E foaf_surname: Siekhaus foaf_workInfoHomepage: http://www.librecat.org/personId=3D224B9E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-8323-8353 bibo_doi: 10.7554/elife.41801 bibo_volume: 8 dct_date: 2019^xs_gYear dct_identifier: - UT:000462530200001 dct_isPartOf: - http://id.crossref.org/issn/2050-084X dct_language: eng dct_publisher: eLife Sciences Publications@ dct_title: A conserved major facilitator superfamily member orchestrates a subset of O-glycosylation to aid macrophage tissue invasion@ ...