---
res:
  bibo_abstract:
  - Cryo-electron tomography (cryo-ET) provides unprecedented insights into the molecular
    constituents of biological environments. In combination with an image processing
    method called subtomogram averaging (STA), detailed 3D structures of biological
    molecules can be obtained in large, irregular macromolecular assemblies or in
    situ, without the need for purification. The contextual meta-information these
    methods also provide, such as a protein’s location within its native environment,
    can then be combined with functional data. This allows the derivation of a detailed
    view on the physiological or pathological roles of proteins from the molecular
    to cellular level. Despite their tremendous potential in in situ structural biology,
    cryo-ET and STA have been restricted by methodological limitations, such as the
    low obtainable resolution. Exciting progress now allows one to reach unprecedented
    resolutions in situ, ranging in optimal cases beyond the nanometer barrier. Here,
    I review current frontiers and future challenges in routinely determining high-resolution
    structures in in situ environments using cryo-ET and STA.@eng
  bibo_authorlist:
  - foaf_Person:
      foaf_givenName: Florian KM
      foaf_name: Schur, Florian KM
      foaf_surname: Schur
      foaf_workInfoHomepage: http://www.librecat.org/personId=48AD8942-F248-11E8-B48F-1D18A9856A87
    orcid: 0000-0003-4790-8078
  bibo_doi: 10.1016/j.sbi.2019.03.018
  bibo_issue: '10'
  bibo_volume: 58
  dct_date: 2019^xs_gYear
  dct_identifier:
  - UT:000494891800004
  dct_isPartOf:
  - http://id.crossref.org/issn/0959-440X
  dct_language: eng
  dct_publisher: Elsevier@
  dct_title: Toward high-resolution in situ structural biology with cryo-electron
    tomography and subtomogram averaging@
...