---
_id: '6343'
abstract:
- lang: eng
  text: Cryo-electron tomography (cryo-ET) provides unprecedented insights into the
    molecular constituents of biological environments. In combination with an image
    processing method called subtomogram averaging (STA), detailed 3D structures of
    biological molecules can be obtained in large, irregular macromolecular assemblies
    or in situ, without the need for purification. The contextual meta-information
    these methods also provide, such as a protein’s location within its native environment,
    can then be combined with functional data. This allows the derivation of a detailed
    view on the physiological or pathological roles of proteins from the molecular
    to cellular level. Despite their tremendous potential in in situ structural biology,
    cryo-ET and STA have been restricted by methodological limitations, such as the
    low obtainable resolution. Exciting progress now allows one to reach unprecedented
    resolutions in situ, ranging in optimal cases beyond the nanometer barrier. Here,
    I review current frontiers and future challenges in routinely determining high-resolution
    structures in in situ environments using cryo-ET and STA.
acknowledgement: The author acknowledges support from IST Austria and the Austrian
  Science Fund (FWF).
article_processing_charge: No
article_type: original
author:
- first_name: Florian KM
  full_name: Schur, Florian KM
  id: 48AD8942-F248-11E8-B48F-1D18A9856A87
  last_name: Schur
  orcid: 0000-0003-4790-8078
citation:
  ama: Schur FK. Toward high-resolution in situ structural biology with cryo-electron
    tomography and subtomogram averaging. <i>Current Opinion in Structural Biology</i>.
    2019;58(10):1-9. doi:<a href="https://doi.org/10.1016/j.sbi.2019.03.018">10.1016/j.sbi.2019.03.018</a>
  apa: Schur, F. K. (2019). Toward high-resolution in situ structural biology with
    cryo-electron tomography and subtomogram averaging. <i>Current Opinion in Structural
    Biology</i>. Elsevier. <a href="https://doi.org/10.1016/j.sbi.2019.03.018">https://doi.org/10.1016/j.sbi.2019.03.018</a>
  chicago: Schur, Florian KM. “Toward High-Resolution in Situ Structural Biology with
    Cryo-Electron Tomography and Subtomogram Averaging.” <i>Current Opinion in Structural
    Biology</i>. Elsevier, 2019. <a href="https://doi.org/10.1016/j.sbi.2019.03.018">https://doi.org/10.1016/j.sbi.2019.03.018</a>.
  ieee: F. K. Schur, “Toward high-resolution in situ structural biology with cryo-electron
    tomography and subtomogram averaging,” <i>Current Opinion in Structural Biology</i>,
    vol. 58, no. 10. Elsevier, pp. 1–9, 2019.
  ista: Schur FK. 2019. Toward high-resolution in situ structural biology with cryo-electron
    tomography and subtomogram averaging. Current Opinion in Structural Biology. 58(10),
    1–9.
  mla: Schur, Florian KM. “Toward High-Resolution in Situ Structural Biology with
    Cryo-Electron Tomography and Subtomogram Averaging.” <i>Current Opinion in Structural
    Biology</i>, vol. 58, no. 10, Elsevier, 2019, pp. 1–9, doi:<a href="https://doi.org/10.1016/j.sbi.2019.03.018">10.1016/j.sbi.2019.03.018</a>.
  short: F.K. Schur, Current Opinion in Structural Biology 58 (2019) 1–9.
date_created: 2019-04-19T11:19:13Z
date_published: 2019-10-01T00:00:00Z
date_updated: 2023-08-25T10:13:31Z
day: '01'
department:
- _id: FlSc
doi: 10.1016/j.sbi.2019.03.018
external_id:
  isi:
  - '000494891800004'
intvolume: '        58'
isi: 1
issue: '10'
language:
- iso: eng
month: '10'
oa_version: None
page: 1-9
publication: Current Opinion in Structural Biology
publication_identifier:
  issn:
  - 0959-440X
publication_status: published
publisher: Elsevier
quality_controlled: '1'
scopus_import: '1'
status: public
title: Toward high-resolution in situ structural biology with cryo-electron tomography
  and subtomogram averaging
type: journal_article
user_id: 4359f0d1-fa6c-11eb-b949-802e58b17ae8
volume: 58
year: '2019'
...