{"publisher":"Springer Nature","date_updated":"2023-08-25T10:33:51Z","publication":"Nature Communications","has_accepted_license":"1","date_created":"2019-05-19T21:59:14Z","ddc":["570"],"file":[{"file_id":"6471","content_type":"application/pdf","checksum":"e214d3e4f8c81e35981583c4569b51b8","file_name":"2019_NatureComm_Chassin.pdf","date_updated":"2020-07-14T12:47:31Z","file_size":1191827,"creator":"dernst","access_level":"open_access","relation":"main_file","date_created":"2019-05-20T07:33:54Z"}],"_id":"6465","isi":1,"article_number":"2013","quality_controlled":"1","oa":1,"user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","author":[{"full_name":"Chassin, Hélène","first_name":"Hélène","last_name":"Chassin"},{"last_name":"Müller","full_name":"Müller, Marius","first_name":"Marius"},{"last_name":"Tigges","first_name":"Marcel","full_name":"Tigges, Marcel"},{"full_name":"Scheller, Leo","first_name":"Leo","last_name":"Scheller"},{"full_name":"Lang, Moritz","first_name":"Moritz","id":"29E0800A-F248-11E8-B48F-1D18A9856A87","last_name":"Lang"},{"last_name":"Fussenegger","full_name":"Fussenegger, Martin","first_name":"Martin"}],"day":"01","tmp":{"legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","image":"/images/cc_by.png"},"oa_version":"Published Version","article_processing_charge":"No","external_id":{"isi":["000466338600006"]},"publication_status":"published","publication_identifier":{"eissn":["20411723"]},"file_date_updated":"2020-07-14T12:47:31Z","month":"05","title":"A modular degron library for synthetic circuits in mammalian cells","department":[{"_id":"CaGu"}],"citation":{"apa":"Chassin, H., Müller, M., Tigges, M., Scheller, L., Lang, M., & Fussenegger, M. (2019). A modular degron library for synthetic circuits in mammalian cells. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-019-09974-5","short":"H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, M. Fussenegger, Nature Communications 10 (2019).","mla":"Chassin, Hélène, et al. “A Modular Degron Library for Synthetic Circuits in Mammalian Cells.” Nature Communications, vol. 10, no. 1, 2013, Springer Nature, 2019, doi:10.1038/s41467-019-09974-5.","ieee":"H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, and M. Fussenegger, “A modular degron library for synthetic circuits in mammalian cells,” Nature Communications, vol. 10, no. 1. Springer Nature, 2019.","chicago":"Chassin, Hélène, Marius Müller, Marcel Tigges, Leo Scheller, Moritz Lang, and Martin Fussenegger. “A Modular Degron Library for Synthetic Circuits in Mammalian Cells.” Nature Communications. Springer Nature, 2019. https://doi.org/10.1038/s41467-019-09974-5.","ista":"Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. 2019. A modular degron library for synthetic circuits in mammalian cells. Nature Communications. 10(1), 2013.","ama":"Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. A modular degron library for synthetic circuits in mammalian cells. Nature Communications. 2019;10(1). doi:10.1038/s41467-019-09974-5"},"date_published":"2019-05-01T00:00:00Z","license":"https://creativecommons.org/licenses/by/4.0/","type":"journal_article","scopus_import":"1","status":"public","intvolume":" 10","issue":"1","volume":10,"language":[{"iso":"eng"}],"year":"2019","abstract":[{"lang":"eng","text":"Tight control over protein degradation is a fundamental requirement for cells to respond rapidly to various stimuli and adapt to a fluctuating environment. Here we develop a versatile, easy-to-handle library of destabilizing tags (degrons) for the precise regulation of protein expression profiles in mammalian cells by modulating target protein half-lives in a predictable manner. Using the well-established tetracycline gene-regulation system as a model, we show that the dynamics of protein expression can be tuned by fusing appropriate degron tags to gene regulators. Next, we apply this degron library to tune a synthetic pulse-generating circuit in mammalian cells. With this toolbox we establish a set of pulse generators with tailored pulse lengths and magnitudes of protein expression. This methodology will prove useful in the functional roles of essential proteins, fine-tuning of gene-expression systems, and enabling a higher complexity in the design of synthetic biological systems in mammalian cells."}],"doi":"10.1038/s41467-019-09974-5","related_material":{"link":[{"relation":"erratum","url":"https://doi.org/10.1038/s41467-023-36111-0"}]}}