{"date_created":"2019-05-19T21:59:14Z","date_updated":"2023-08-25T10:33:51Z","volume":10,"author":[{"last_name":"Chassin","first_name":"Hélène","full_name":"Chassin, Hélène"},{"first_name":"Marius","last_name":"Müller","full_name":"Müller, Marius"},{"full_name":"Tigges, Marcel","last_name":"Tigges","first_name":"Marcel"},{"full_name":"Scheller, Leo","first_name":"Leo","last_name":"Scheller"},{"id":"29E0800A-F248-11E8-B48F-1D18A9856A87","last_name":"Lang","first_name":"Moritz","full_name":"Lang, Moritz"},{"full_name":"Fussenegger, Martin","last_name":"Fussenegger","first_name":"Martin"}],"related_material":{"link":[{"url":"https://doi.org/10.1038/s41467-023-36111-0","relation":"erratum"}]},"publication_status":"published","publisher":"Springer Nature","department":[{"_id":"CaGu"}],"year":"2019","file_date_updated":"2020-07-14T12:47:31Z","article_number":"2013","language":[{"iso":"eng"}],"doi":"10.1038/s41467-019-09974-5","isi":1,"quality_controlled":"1","tmp":{"name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode","short":"CC BY (4.0)","image":"/images/cc_by.png"},"external_id":{"isi":["000466338600006"]},"oa":1,"month":"05","publication_identifier":{"eissn":["20411723"]},"oa_version":"Published Version","file":[{"date_created":"2019-05-20T07:33:54Z","date_updated":"2020-07-14T12:47:31Z","checksum":"e214d3e4f8c81e35981583c4569b51b8","file_id":"6471","relation":"main_file","creator":"dernst","file_size":1191827,"content_type":"application/pdf","file_name":"2019_NatureComm_Chassin.pdf","access_level":"open_access"}],"title":"A modular degron library for synthetic circuits in mammalian cells","status":"public","ddc":["570"],"intvolume":" 10","user_id":"4359f0d1-fa6c-11eb-b949-802e58b17ae8","_id":"6465","abstract":[{"text":"Tight control over protein degradation is a fundamental requirement for cells to respond rapidly to various stimuli and adapt to a fluctuating environment. Here we develop a versatile, easy-to-handle library of destabilizing tags (degrons) for the precise regulation of protein expression profiles in mammalian cells by modulating target protein half-lives in a predictable manner. Using the well-established tetracycline gene-regulation system as a model, we show that the dynamics of protein expression can be tuned by fusing appropriate degron tags to gene regulators. Next, we apply this degron library to tune a synthetic pulse-generating circuit in mammalian cells. With this toolbox we establish a set of pulse generators with tailored pulse lengths and magnitudes of protein expression. This methodology will prove useful in the functional roles of essential proteins, fine-tuning of gene-expression systems, and enabling a higher complexity in the design of synthetic biological systems in mammalian cells.","lang":"eng"}],"issue":"1","type":"journal_article","date_published":"2019-05-01T00:00:00Z","publication":"Nature Communications","citation":{"chicago":"Chassin, Hélène, Marius Müller, Marcel Tigges, Leo Scheller, Moritz Lang, and Martin Fussenegger. “A Modular Degron Library for Synthetic Circuits in Mammalian Cells.” Nature Communications. Springer Nature, 2019. https://doi.org/10.1038/s41467-019-09974-5.","short":"H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, M. Fussenegger, Nature Communications 10 (2019).","mla":"Chassin, Hélène, et al. “A Modular Degron Library for Synthetic Circuits in Mammalian Cells.” Nature Communications, vol. 10, no. 1, 2013, Springer Nature, 2019, doi:10.1038/s41467-019-09974-5.","ieee":"H. Chassin, M. Müller, M. Tigges, L. Scheller, M. Lang, and M. Fussenegger, “A modular degron library for synthetic circuits in mammalian cells,” Nature Communications, vol. 10, no. 1. Springer Nature, 2019.","apa":"Chassin, H., Müller, M., Tigges, M., Scheller, L., Lang, M., & Fussenegger, M. (2019). A modular degron library for synthetic circuits in mammalian cells. Nature Communications. Springer Nature. https://doi.org/10.1038/s41467-019-09974-5","ista":"Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. 2019. A modular degron library for synthetic circuits in mammalian cells. Nature Communications. 10(1), 2013.","ama":"Chassin H, Müller M, Tigges M, Scheller L, Lang M, Fussenegger M. A modular degron library for synthetic circuits in mammalian cells. Nature Communications. 2019;10(1). doi:10.1038/s41467-019-09974-5"},"day":"01","has_accepted_license":"1","article_processing_charge":"No","scopus_import":"1"}