{"abstract":[{"text":"T cells are actively scanning pMHC-presenting cells in lymphoid organs and nonlymphoid tissues (NLTs) with divergent topologies and confinement. How the T cell actomyosin cytoskeleton facilitates this task in distinct environments is incompletely understood. Here, we show that lack of Myosin IXb (Myo9b), a negative regulator of the small GTPase Rho, led to increased Rho-GTP levels and cell surface stiffness in primary T cells. Nonetheless, intravital imaging revealed robust motility of Myo9b−/− CD8+ T cells in lymphoid tissue and similar expansion and differentiation during immune responses. In contrast, accumulation of Myo9b−/− CD8+ T cells in NLTs was strongly impaired. Specifically, Myo9b was required for T cell crossing of basement membranes, such as those which are present between dermis and epidermis. As consequence, Myo9b−/− CD8+ T cells showed impaired control of skin infections. In sum, we show that Myo9b is critical for the CD8+ T cell adaptation from lymphoid to NLT surveillance and the establishment of protective tissue–resident T cell populations.","lang":"eng"}],"publisher":"Rockefeller University Press","article_processing_charge":"No","issue":"7","language":[{"iso":"eng"}],"user_id":"c635000d-4b10-11ee-a964-aac5a93f6ac1","file_date_updated":"2020-07-14T12:47:32Z","doi":"10.1084/jem.20170896","scopus_import":"1","citation":{"short":"F. Moalli, X. Ficht, P. Germann, M. Vladymyrov, B. Stolp, I. de Vries, R. Lyck, J. Balmer, A. Fiocchi, M. Kreutzfeldt, D. Merkler, M. Iannacone, A. Ariga, M.H. Stoffel, J. Sharpe, M. Bähler, M.K. Sixt, A. Diz-Muñoz, J.V. Stein, The Journal of Experimental Medicine 2015 (2018) 1869–1890.","apa":"Moalli, F., Ficht, X., Germann, P., Vladymyrov, M., Stolp, B., de Vries, I., … Stein, J. V. (2018). The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells. <i>The Journal of Experimental Medicine</i>. Rockefeller University Press. <a href=\"https://doi.org/10.1084/jem.20170896\">https://doi.org/10.1084/jem.20170896</a>","ista":"Moalli F, Ficht X, Germann P, Vladymyrov M, Stolp B, de Vries I, Lyck R, Balmer J, Fiocchi A, Kreutzfeldt M, Merkler D, Iannacone M, Ariga A, Stoffel MH, Sharpe J, Bähler M, Sixt MK, Diz-Muñoz A, Stein JV. 2018. The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells. The Journal of Experimental Medicine. 2015(7), 1869–1890.","mla":"Moalli, Federica, et al. “The Rho Regulator Myosin IXb Enables Nonlymphoid Tissue Seeding of Protective CD8+T Cells.” <i>The Journal of Experimental Medicine</i>, vol. 2015, no. 7, Rockefeller University Press, 2018, pp. 1869–1890, doi:<a href=\"https://doi.org/10.1084/jem.20170896\">10.1084/jem.20170896</a>.","chicago":"Moalli, Federica, Xenia Ficht, Philipp Germann, Mykhailo Vladymyrov, Bettina Stolp, Ingrid de Vries, Ruth Lyck, et al. “The Rho Regulator Myosin IXb Enables Nonlymphoid Tissue Seeding of Protective CD8+T Cells.” <i>The Journal of Experimental Medicine</i>. Rockefeller University Press, 2018. <a href=\"https://doi.org/10.1084/jem.20170896\">https://doi.org/10.1084/jem.20170896</a>.","ama":"Moalli F, Ficht X, Germann P, et al. The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells. <i>The Journal of Experimental Medicine</i>. 2018;2015(7):1869–1890. doi:<a href=\"https://doi.org/10.1084/jem.20170896\">10.1084/jem.20170896</a>","ieee":"F. Moalli <i>et al.</i>, “The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells,” <i>The Journal of Experimental Medicine</i>, vol. 2015, no. 7. Rockefeller University Press, pp. 1869–1890, 2018."},"day":"06","status":"public","publication_identifier":{"eissn":["1540-9538"],"issn":["0022-1007"]},"date_published":"2018-06-06T00:00:00Z","quality_controlled":"1","external_id":{"isi":["000440822900011"]},"publication":"The Journal of Experimental Medicine","has_accepted_license":"1","file":[{"file_size":3841660,"checksum":"86ae5331f9bfced9a6358a790a04bef4","access_level":"open_access","content_type":"application/pdf","date_created":"2019-05-28T12:40:05Z","file_id":"6498","relation":"main_file","file_name":"2018_rupress_Moalli.pdf","creator":"kschuh","date_updated":"2020-07-14T12:47:32Z"}],"intvolume":"      2015","tmp":{"name":"Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)","short":"CC BY-NC-SA (4.0)","image":"/images/cc_by_nc_sa.png","legal_code_url":"https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode"},"isi":1,"oa_version":"Published Version","oa":1,"publication_status":"published","year":"2018","type":"journal_article","author":[{"full_name":"Moalli, Federica","last_name":"Moalli","first_name":"Federica"},{"first_name":"Xenia","last_name":"Ficht","full_name":"Ficht, Xenia"},{"first_name":"Philipp","last_name":"Germann","full_name":"Germann, Philipp"},{"full_name":"Vladymyrov, Mykhailo","last_name":"Vladymyrov","first_name":"Mykhailo"},{"first_name":"Bettina","last_name":"Stolp","full_name":"Stolp, Bettina"},{"id":"4C7D837E-F248-11E8-B48F-1D18A9856A87","first_name":"Ingrid","last_name":"de Vries","full_name":"de Vries, Ingrid"},{"last_name":"Lyck","full_name":"Lyck, Ruth","first_name":"Ruth"},{"last_name":"Balmer","full_name":"Balmer, Jasmin","first_name":"Jasmin"},{"first_name":"Amleto","last_name":"Fiocchi","full_name":"Fiocchi, Amleto"},{"last_name":"Kreutzfeldt","full_name":"Kreutzfeldt, Mario","first_name":"Mario"},{"last_name":"Merkler","full_name":"Merkler, Doron","first_name":"Doron"},{"first_name":"Matteo","last_name":"Iannacone","full_name":"Iannacone, Matteo"},{"first_name":"Akitaka","last_name":"Ariga","full_name":"Ariga, Akitaka"},{"last_name":"Stoffel","full_name":"Stoffel, Michael H.","first_name":"Michael H."},{"last_name":"Sharpe","full_name":"Sharpe, James","first_name":"James"},{"full_name":"Bähler, Martin","last_name":"Bähler","first_name":"Martin"},{"full_name":"Sixt, Michael K","last_name":"Sixt","id":"41E9FBEA-F248-11E8-B48F-1D18A9856A87","first_name":"Michael K","orcid":"0000-0002-6620-9179"},{"last_name":"Diz-Muñoz","full_name":"Diz-Muñoz, Alba","first_name":"Alba"},{"full_name":"Stein, Jens V.","last_name":"Stein","first_name":"Jens V."}],"ddc":["570"],"title":"The Rho regulator Myosin IXb enables nonlymphoid tissue seeding of protective CD8+T cells","_id":"6497","department":[{"_id":"MiSi"}],"date_created":"2019-05-28T12:36:47Z","page":"1869–1890","date_updated":"2023-09-19T14:52:08Z","month":"06","volume":2015}