--- res: bibo_abstract: - Optogenetics enables the spatio-temporally precise control of cell and animal behavior. Many optogenetic tools are driven by light-controlled protein–protein interactions (PPIs) that are repurposed from natural light-sensitive domains (LSDs). Applying light-controlled PPIs to new target proteins is challenging because it is difficult to predict which of the many available LSDs, if any, will yield robust light regulation. As a consequence, fusion protein libraries need to be prepared and tested, but methods and platforms to facilitate this process are currently not available. Here, we developed a genetic engineering strategy and vector library for the rapid generation of light-controlled PPIs. The strategy permits fusing a target protein to multiple LSDs efficiently and in two orientations. The public and expandable library contains 29 vectors with blue, green or red light-responsive LSDs, many of which have been previously applied ex vivo and in vivo. We demonstrate the versatility of the approach and the necessity for sampling LSDs by generating light-activated caspase-9 (casp9) enzymes. Collectively, this work provides a new resource for optical regulation of a broad range of target proteins in cell and developmental biology.@eng bibo_authorlist: - foaf_Person: foaf_givenName: Alexandra-Madelaine foaf_name: Tichy, Alexandra-Madelaine foaf_surname: Tichy foaf_workInfoHomepage: http://www.librecat.org/personId=29D8BB2C-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Elliot J. foaf_name: Gerrard, Elliot J. foaf_surname: Gerrard - foaf_Person: foaf_givenName: Julien M.D. foaf_name: Legrand, Julien M.D. foaf_surname: Legrand - foaf_Person: foaf_givenName: Robin M. foaf_name: Hobbs, Robin M. foaf_surname: Hobbs - foaf_Person: foaf_givenName: Harald L foaf_name: Janovjak, Harald L foaf_surname: Janovjak foaf_workInfoHomepage: http://www.librecat.org/personId=33BA6C30-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-8023-9315 bibo_doi: 10.1016/j.jmb.2019.05.033 bibo_issue: '17' bibo_volume: 431 dct_date: 2019^xs_gYear dct_identifier: - UT:000482872100002 dct_isPartOf: - http://id.crossref.org/issn/00222836 - http://id.crossref.org/issn/10898638 dct_language: eng dct_publisher: Elsevier@ dct_title: Engineering strategy and vector library for the rapid generation of modular light-controlled protein–protein interactions@ ...