---
_id: '660'
abstract:
- lang: eng
  text: Growing microtubules are protected from depolymerization by the presence of
    a GTP or GDP/Pi cap. End-binding proteins of the EB1 family bind to the stabilizing
    cap, allowing monitoring of its size in real time. The cap size has been shown
    to correlate with instantaneous microtubule stability. Here we have quantitatively
    characterized the properties of cap size fluctuations during steadystate growth
    and have developed a theory predicting their timescale and amplitude from the
    kinetics of microtubule growth and cap maturation. In contrast to growth speed
    fluctuations, cap size fluctuations show a characteristic timescale, which is
    defined by the lifetime of the cap sites. Growth fluctuations affect the amplitude
    of cap size fluctuations; however, cap size does not affect growth speed, indicating
    that microtubules are far from instability during most of their time of growth.
    Our theory provides the basis for a quantitative understanding of microtubule
    stability fluctuations during steady-state growth.
acknowledgement: We thank Philippe Cluzel for helpful discussions and Gunnar Pruessner
  for data analysis advice. This work was supported by the Francis Crick Institute,
  which receives its core funding from Cancer Research UK Grant FC001163, Medical
  Research Council Grant FC001163, and Wellcome Trust Grant FC001163. This work was
  also supported by European Research Council Advanced Grant Project 323042 (to C.D.
  and T.S.).
article_processing_charge: No
author:
- first_name: Jamie
  full_name: Rickman, Jamie
  last_name: Rickman
- first_name: Christian F
  full_name: Düllberg, Christian F
  id: 459064DC-F248-11E8-B48F-1D18A9856A87
  last_name: Düllberg
  orcid: 0000-0001-6335-9748
- first_name: Nicholas
  full_name: Cade, Nicholas
  last_name: Cade
- first_name: Lewis
  full_name: Griffin, Lewis
  last_name: Griffin
- first_name: Thomas
  full_name: Surrey, Thomas
  last_name: Surrey
citation:
  ama: Rickman J, Düllberg CF, Cade N, Griffin L, Surrey T. Steady state EB cap size
    fluctuations are determined by stochastic microtubule growth and maturation. <i>PNAS</i>.
    2017;114(13):3427-3432. doi:<a href="https://doi.org/10.1073/pnas.1620274114">10.1073/pnas.1620274114</a>
  apa: Rickman, J., Düllberg, C. F., Cade, N., Griffin, L., &#38; Surrey, T. (2017).
    Steady state EB cap size fluctuations are determined by stochastic microtubule
    growth and maturation. <i>PNAS</i>. National Academy of Sciences. <a href="https://doi.org/10.1073/pnas.1620274114">https://doi.org/10.1073/pnas.1620274114</a>
  chicago: Rickman, Jamie, Christian F Düllberg, Nicholas Cade, Lewis Griffin, and
    Thomas Surrey. “Steady State EB Cap Size Fluctuations Are Determined by Stochastic
    Microtubule Growth and Maturation.” <i>PNAS</i>. National Academy of Sciences,
    2017. <a href="https://doi.org/10.1073/pnas.1620274114">https://doi.org/10.1073/pnas.1620274114</a>.
  ieee: J. Rickman, C. F. Düllberg, N. Cade, L. Griffin, and T. Surrey, “Steady state
    EB cap size fluctuations are determined by stochastic microtubule growth and maturation,”
    <i>PNAS</i>, vol. 114, no. 13. National Academy of Sciences, pp. 3427–3432, 2017.
  ista: Rickman J, Düllberg CF, Cade N, Griffin L, Surrey T. 2017. Steady state EB
    cap size fluctuations are determined by stochastic microtubule growth and maturation.
    PNAS. 114(13), 3427–3432.
  mla: Rickman, Jamie, et al. “Steady State EB Cap Size Fluctuations Are Determined
    by Stochastic Microtubule Growth and Maturation.” <i>PNAS</i>, vol. 114, no. 13,
    National Academy of Sciences, 2017, pp. 3427–32, doi:<a href="https://doi.org/10.1073/pnas.1620274114">10.1073/pnas.1620274114</a>.
  short: J. Rickman, C.F. Düllberg, N. Cade, L. Griffin, T. Surrey, PNAS 114 (2017)
    3427–3432.
date_created: 2018-12-11T11:47:46Z
date_published: 2017-03-28T00:00:00Z
date_updated: 2025-09-11T07:08:20Z
day: '28'
department:
- _id: MaLo
doi: 10.1073/pnas.1620274114
external_id:
  isi:
  - '000397607300065'
  pmid:
  - '28280102'
intvolume: '       114'
isi: 1
issue: '13'
language:
- iso: eng
main_file_link:
- open_access: '1'
  url: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5380103/
month: '03'
oa: 1
oa_version: Submitted Version
page: 3427 - 3432
pmid: 1
publication: PNAS
publication_identifier:
  issn:
  - 0027-8424
publication_status: published
publisher: National Academy of Sciences
publist_id: '7073'
quality_controlled: '1'
scopus_import: '1'
status: public
title: Steady state EB cap size fluctuations are determined by stochastic microtubule
  growth and maturation
type: journal_article
user_id: 317138e5-6ab7-11ef-aa6d-ffef3953e345
volume: 114
year: '2017'
...