Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity Bergmiller, Tobias ; https://orcid.org/0000-0001-5396-4346 Andersson, Anna M ; https://orcid.org/0000-0003-2912-6769 Tomasek, Kathrin ; https://orcid.org/0000-0003-3768-877X Balleza, Enrique Kiviet, Daniel Hauschild, Robert ; https://orcid.org/0000-0001-9843-3522 Tkacik, Gasper ; https://orcid.org/0000-0002-6699-1455 Guet, Calin C ; https://orcid.org/0000-0001-6220-2052 The molecular mechanisms underlying phenotypic variation in isogenic bacterial populations remain poorly understood.We report that AcrAB-TolC, the main multidrug efflux pump of Escherichia coli, exhibits a strong partitioning bias for old cell poles by a segregation mechanism that is mediated by ternary AcrAB-TolC complex formation. Mother cells inheriting old poles are phenotypically distinct and display increased drug efflux activity relative to daughters. Consequently, we find systematic and long-lived growth differences between mother and daughter cells in the presence of subinhibitory drug concentrations. A simple model for biased partitioning predicts a population structure of long-lived and highly heterogeneous phenotypes. This straightforward mechanism of generating sustained growth rate differences at subinhibitory antibiotic concentrations has implications for understanding the emergence of multidrug resistance in bacteria. American Association for the Advancement of Science 2017 info:eu-repo/semantics/article doc-type:article text http://purl.org/coar/resource_type/c_2df8fbb1 https://research-explorer.ista.ac.at/record/665 Bergmiller T, Andersson AM, Tomasek K, et al. Biased partitioning of the multidrug efflux pump AcrAB TolC underlies long lived phenotypic heterogeneity. <i>Science</i>. 2017;356(6335):311-315. doi:<a href="https://doi.org/10.1126/science.aaf4762">10.1126/science.aaf4762</a> eng info:eu-repo/semantics/altIdentifier/doi/10.1126/science.aaf4762 info:eu-repo/semantics/altIdentifier/issn/0036-8075 info:eu-repo/semantics/altIdentifier/wos/000399540100060 info:eu-repo/semantics/closedAccess