--- res: bibo_abstract: - 'Gene regulatory networks evolve through rewiring of individual components—that is, through changes in regulatory connections. However, the mechanistic basis of regulatory rewiring is poorly understood. Using a canonical gene regulatory system, we quantify the properties of transcription factors that determine the evolutionary potential for rewiring of regulatory connections: robustness, tunability and evolvability. In vivo repression measurements of two repressors at mutated operator sites reveal their contrasting evolutionary potential: while robustness and evolvability were positively correlated, both were in trade-off with tunability. Epistatic interactions between adjacent operators alleviated this trade-off. A thermodynamic model explains how the differences in robustness, tunability and evolvability arise from biophysical characteristics of repressor–DNA binding. The model also uncovers that the energy matrix, which describes how mutations affect repressor–DNA binding, encodes crucial information about the evolutionary potential of a repressor. The biophysical determinants of evolutionary potential for regulatory rewiring constitute a mechanistic framework for understanding network evolution.@eng' bibo_authorlist: - foaf_Person: foaf_givenName: Claudia foaf_name: Igler, Claudia foaf_surname: Igler foaf_workInfoHomepage: http://www.librecat.org/personId=46613666-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Mato foaf_name: Lagator, Mato foaf_surname: Lagator foaf_workInfoHomepage: http://www.librecat.org/personId=345D25EC-F248-11E8-B48F-1D18A9856A87 - foaf_Person: foaf_givenName: Gasper foaf_name: Tkacik, Gasper foaf_surname: Tkacik foaf_workInfoHomepage: http://www.librecat.org/personId=3D494DCA-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-6699-1455 - foaf_Person: foaf_givenName: Jonathan P foaf_name: Bollback, Jonathan P foaf_surname: Bollback foaf_workInfoHomepage: http://www.librecat.org/personId=2C6FA9CC-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0002-4624-4612 - foaf_Person: foaf_givenName: Calin C foaf_name: Guet, Calin C foaf_surname: Guet foaf_workInfoHomepage: http://www.librecat.org/personId=47F8433E-F248-11E8-B48F-1D18A9856A87 orcid: 0000-0001-6220-2052 bibo_doi: 10.1038/s41559-018-0651-y bibo_issue: '10' bibo_volume: 2 dct_date: 2018^xs_gYear dct_identifier: - UT:000447947600021 dct_language: eng dct_publisher: Nature Publishing Group@ dct_title: Evolutionary potential of transcription factors for gene regulatory rewiring@ ...